eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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4/2020
vol. 101
 
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abstract:
RESEARCH PAPERS

Chemical composition and cellulase-assisted extraction of total saponins from Gomphrena celosioides Mart.

Nguyen Thi Hoang Yen
1
,
Le Pham Tan Quoc
1

1.
Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Vietnam
BioTechnologia vol. 101 (4) C pp. 361–367 C 2020
Online publish date: 2021/01/04
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The major purpose of the present study was to determine the chemical composition of dried Gomphrena celosioides Mart., including crude protein, lipid, total sugar, reducing sugar, ash, crude fiber, moisture, crude pectin, and total saponins in particular. This plant contains a large percentage of total saponins, which are widely used in food technology and pharmaceutical industries. In our study, the conditions for the extraction of total saponins from the plant were optimized, with special focus on cellulase concentration, pH, hydrolyzation time, and temperature. The highest amount of total saponins content (TSC) was 1.550±0.016% under optimal extraction conditions (cellulase concentration: 0.7% (v/w), hydrolyzation time: 4 h, hydrolyzation temperature: 60EC, and pH value: 5). Accordingly, the total saponins from G. celosioides Mart. were easily extracted.
keywords:

bioactive compounds, composition, enzyme, extraction, Gomphrena celosioides Mart

 
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