eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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1/2021
vol. 102
 
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abstract:
RESEARCH PAPERS

Effects of different hormonal treatments on growth parameters and secondary metabolite production in organ culture of Hyssopus officinalis L.

Hanieh Mohajjel Shoja
1
,
Hengameh Kharrati Shishavani
1

1.
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
BioTechnologia vol. 102 (1) C pp. 33–41 C 2021
Online publish date: 2021/03/31
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Hyssopus officinalis L. is a medicinal plant containing valuable phytochemicals and is used extensively in the pharma¬ceutical industry. As this plant naturally contains low levels of these metabolites, plant cell and tissue cul¬ture technologies are used to produce them in large volumes. The present study aimed first to evaluate the effects of different types of cytokinins, including benzyl adenine (BA), kinetin (KIN), and thidiazuron (TDZ), at 1 ppm concentration in the culture medium on growth parameters and production of secondary metabolites and photo¬synthetic pigments in organ culture of H. officinalis; in the second step, the study investigated the effect of dif¬ferent concentrations (0, 0.2, 1, 1.5, and 2 ppm) of the most effective type of cytokinin. The results showed that TDZ was the optimal type of growth regulator compared to BA and KIN as it showed a positive influence on the studied traits; furthermore, its highest concentration of 2 ppm in culture medium was determined to be the best one that enabled the highest production of secondary metabolites and photosynthetic pigments. We conclude that the presence of growth regulators can strongly influence the growth and development of plants in tissue culture conditions.
keywords:

growth parameters, Hyssopus officinalis, photosynthetic pigment, secondary metabolite, thidiazuron

 
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