Impact of Enterococcus italicus ONU547 on the growth
and acclimatization of micropropagated Rubus fruticosus L.
and Paulownia tomentosa Steud. plants to ex vitro conditions

Nadiia Tytarenko; Nataliia Tesliuk; Andrii Merlich; Thomas Haertle; Volodymyr Ivanytsia

doi:10.5114/bta.2023.130732

eISSN: 2353-9461
ISSN: 0860-7796

BioTechnologia

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abstract:

RESEARCH PAPERS

Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

Nadiia Tytarenko

¹

,

Nataliia Tesliuk

¹

,

Andrii Merlich

¹

,

Thomas Haertle

²

,

Volodymyr Ivanytsia

¹

Odesa I.I. Mechnikov National University, Odesa, Ukraine
Institut National des la Recherche Agronomique, Nantes, France

BioTechnologia vol. 104(3) ∙ 301-313 ∙ 2023

DOI: https://doi.org/10.5114/bta.2023.130732

Online publish date: 2023/09/25

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AMA

Tytarenko N, Tesliuk N, Merlich A, Haertle T, Ivanytsia V. Impact of Enterococcus italicus ONU547 on the growth
and acclimatization of micropropagated Rubus fruticosus L.
and Paulownia tomentosa Steud. plants to ex vitro conditions. BioTechnologia. 2023;104(3):301-313. doi:10.5114/bta.2023.130732.

APA

Tytarenko, N., Tesliuk, N., Merlich, A., Haertle, T.,  & Ivanytsia, V. (2023). Impact of Enterococcus italicus ONU547 on the growth
and acclimatization of micropropagated Rubus fruticosus L.
and Paulownia tomentosa Steud. plants to ex vitro conditions. BioTechnologia, 104(3), 301-313. https://doi.org/10.5114/bta.2023.130732

Chicago

Tytarenko, Nadiia, Nataliia Tesliuk, Andrii Merlich, Thomas Haertle,  and Volodymyr Ivanytsia. 2023. "Impact of Enterococcus italicus ONU547 on the growth
and acclimatization of micropropagated Rubus fruticosus L.
and Paulownia tomentosa Steud. plants to ex vitro conditions". BioTechnologia 104 (3): 301-313. doi:10.5114/bta.2023.130732.

Harvard

Tytarenko, N., Tesliuk, N., Merlich, A., Haertle, T.,  and Ivanytsia, V. (2023). Impact of Enterococcus italicus ONU547 on the growth
and acclimatization of micropropagated Rubus fruticosus L.
and Paulownia tomentosa Steud. plants to ex vitro conditions. BioTechnologia, 104(3), pp.301-313. https://doi.org/10.5114/bta.2023.130732

MLA

Tytarenko, Nadiia et al. "Impact of Enterococcus italicus ONU547 on the growth
and acclimatization of micropropagated Rubus fruticosus L.
and Paulownia tomentosa Steud. plants to ex vitro conditions." BioTechnologia, vol. 104, no. 3, 2023, pp. 301-313. doi:10.5114/bta.2023.130732.

Vancouver

Tytarenko N, Tesliuk N, Merlich A, Haertle T, Ivanytsia V. Impact of Enterococcus italicus ONU547 on the growth
and acclimatization of micropropagated Rubus fruticosus L.
and Paulownia tomentosa Steud. plants to ex vitro conditions. BioTechnologia. 2023;104(3):301-313. doi:10.5114/bta.2023.130732.

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Clonal micropropagation is an effective method for plant reproduction, applicable in both scientific and industrial domains. However, a significant number of microclones are lost during the ex vitro acclimatization process. To address this, the introduction of beneficial microorganisms into the rhizosphere of micropropagated plants could have a positive effect on the survival rates and external characteristics of acclimatized plantlets. The aim of this study was to determine the protective and growth-promoting potential of Enterococcus italicus ONU547 and its effect on micropropagated plants during acclimatization. The antagonistic activity of the bacteria was determined using the agar block method. Lepidium sativum L. seeds were inoculated with bacterial suspensions at concentrations of 10⁶, 10⁷, and 10⁸ CFU/ml. Subsequently, the roots of the microclones were treated with suspensions of 10⁶ and 10⁷ CFU/ml, and biometric characteristics were measured. The results demonstrated antagonistic properties against various phytopathogenic fungi, including Aspergillus niger, Cladosporium cladosporioides, Alternaria alternata, Alternaria tenuissima, Rhizoctonia cerealis, Penicillium expansum, and Paecilomyces variotii. Inoculation of L. sativum L. seeds resulted in improved germination rates, increased root numbers, and enhanced root and shoot lengths. Similarly, the effects of the studied bacteria on Rubus fruticosus L. and Paulownia tomentosa Steud. during the acclimatization stage led to higher survival rates, increased shoot lengths, greater node numbers, and larger leaf areas. A concentration of 10⁷ CFU/ml was identified as optimal for inoculating the microclones. The findings indicate that E. italicus ONU547 holds promise for the inoculation of micropropagated plants during the acclimatization process. Further research is recommended to establish the specific interaction mechanisms between these bacteria and plants.

keywords:

Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

Nadiia Tytarenko 1 , Nataliia Tesliuk 1 , Andrii Merlich 1 , Thomas Haertle 2 , Volodymyr Ivanytsia 1

Paulownia tomentosa, Rubus fruticosus, ex vitro acclimatization, Enterococcus italicus, plant growth-promoting bacteria, antagonism

Nadiia Tytarenko

¹

,

Nataliia Tesliuk

¹

,

Andrii Merlich

¹

,

Thomas Haertle

²

,

Volodymyr Ivanytsia

¹