eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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3/2019
vol. 100
 
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abstract:
RESEARCH PAPERS

Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)

Prabhu Thangavel
,
Sisubalan Natarajan
,
Velmurugam Shanmugam
,
Sheik Mohamed Sulaiman
,
Ravikumar Ramasamy

BioTechnologia vol. 100 (3) C pp. 263–272 C 2019
Online publish date: 2019/09/26
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The present study attempts to explain a mass culture protocol for large scale multiple shoot bud regeneration from nodal explants of Citrullus colocynthis L. The highest shoot bud induction (94.9%) without attaining callus was observed in Murashige and Skoog’s (MS) medium supplemented with benzyl amino purine (BA)in which the combination of benzyl amino purine BAP (1.0 mg/l), kinetin (0.5 mg/l), gibberellic acid (1.5 mg/l) produced more shoots/explants. This study strongly suggests that the pre-eminence of BA and other cytokinins in combination with GA3 was found to be essential for a swift multiple shoot bud induction as well as an enhanced rate of shoot proliferation in C. colocynthis. A full-strength MS medium, supplemented with indole 3 butyric acid IBA (1.5 mg/l) combined with BA (1.5 mg/l), had the maximum rooting response and the regenerated plantlets were successfully established in the field with an 80% survival rate.
keywords:

nodal explants, surface sterilization, mercuric chloride, shoot bud, BA, multiple shoot, IBA, GA3, rooting

 
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