eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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4/2019
vol. 100
 
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abstract:
RESEARCH PAPERS

Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

Jong T. Kiun
,
Tan S. M. Amelia
,
Kai Hee Huong
,
Al-Ashraf Abdullah Amirul
,
Kesaven Bhubalan

BioTechnologia vol. 100 (4) C pp. 359–371 C 2019
Online publish date: 2019/12/25
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Polyhydroxyalkanoate (PHA) is a microbial storage polymer that is naturally produced by certain bacteria. This is the first study on the ability of this particular species Massilia haematophila to synthesize a PHA copolymer containing 3-hydroxyvalerate (3HV) monomer. Using the statistical design on Massilia haematophila UMTKB-2, this study highlights the optimization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), copolymer production for shaken-flask cultivation. Moreover, the mechanical and thermal features of the polymers were determined. The production of P(3HB-co-3HV) by Massilia haematophila UMTKB-2 using optimal conditions provided by response surface methodology (RSM) yielded 5.0 g/l of P(3HB-co-7 mol% 3HV), which was higher than the value obtained from unoptimized conditions such as 4.40 g/l of P(3HB-co-4mol% 3HV). This result showed a 14% increase in copolymer concentration and a two-fold increase in 3HV composition. In this study, the P(3HB-co-3HV) synthesized was determined as a block copolymer and its thermal properties were better than P(3HB). Using RSM, the optimization conditions were successfully obtained for this bacterium, and this result is a starting platform for additional studies of a larger scaled PHA production from Massilia haematophila UMTKB-2 using bioreactors.
keywords:

3-hydroxyvalerate, biorenewable, Massilia haematophila, optimization, polyhydroxyalkanoate, response surface methodology

 
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