2/2011
vol. 92
abstract:
PERSPECTIVES High throughput protein production
BioTechnologia vol. 92(2) C pp. 119-131 C 2011
Online publish date: 2014/10/28
PlumX metrics:
Research in the relationship between the architecture and function of proteins aims to understand the mechanism
of proteins folding and their activity at the atomic level. This knowledge leads to a progress in new technologies
that allow simultaneous production of numerous proteins on a large scale – high throughput protein production
(HTPP) methods. The HTPP methods allow parallel processing of multiple samples and are also important for
the determination of optimal protein production procedure, where usually combinations of different conditions
have to be tested. This article describes available cloning, expression, and purification approaches that may be
used in a high throughput and parallel manner. Additionally, the implementation of automation facilities is briefly
outlined.
keywords:
protein, production, high throughput, structural genomics
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