eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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2/2011
vol. 92
 
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abstract:

PERSPECTIVES
High throughput protein production

Aleksander Tworak
,
Jan Podkowiński
,
Marek Figlerowicz

BioTechnologia vol. 92(2) C pp. 119-131 C 2011
Online publish date: 2014/10/28
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Research in the relationship between the architecture and function of proteins aims to understand the mechanism

of proteins folding and their activity at the atomic level. This knowledge leads to a progress in new technologies

that allow simultaneous production of numerous proteins on a large scale – high throughput protein production

(HTPP) methods. The HTPP methods allow parallel processing of multiple samples and are also important for

the determination of optimal protein production procedure, where usually combinations of different conditions

have to be tested. This article describes available cloning, expression, and purification approaches that may be

used in a high throughput and parallel manner. Additionally, the implementation of automation facilities is briefly

outlined.
keywords:

protein, production, high throughput, structural genomics

 
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