eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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4/2020
vol. 101
 
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abstract:
RESEARCH PAPERS

Phytochemical composition and antibacterial activity of ethanolic extract from trunk of Rothmannia wittii (Craib) Bremek. (Rubiaceae) newly found in Vietnam

Hong Thien Van
1
,
Tai Thu Nguyen
1
,
Hoai Thuong Thi Ton
1
,
Kim Truc Thi Dinh
1
,
Van Son Le
2
,
Tan Viet Pham
1
,
Gia Buu Tran
1

1.
Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Vietnam
2.
Binh Chau-Phuoc Buu Nature Reserve, Bung Rieng Ward, Ba Ria-Vung Tau Province, Vietnam
BioTechnologia vol. 101 (4) C pp. 301–307 C 2020
Online publish date: 2021/01/04
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Rothmannia wittii, which is found in Binh Chau-Phuoc Buu Nature Reserve, Bung Rieng ward, Xuyen Moc District, Ba Ria-Vung Tau Province, Vietnam, is a rare species of Rubiaceae family. The present study aimed to determine the phytochemical composition and antimicrobial activity of the ethanolic extract of the R. wittii trunk. A total of 10 compounds were identified by liquid chromatography-mass spectrophotometry (LC-MS), including 1) benzene, 1-(1,5-dimethyl-4- hexenyl)-4-methyl, 2) diphenylsulfone, 3) 6β-hydroxy-10-O-acetylgenipin, 4) cinnamyl- cinnamate, 5) genipin, 6) megastigmatrienone, 7) ethyl p-methoxy-trans-cinnamate, 8) rothalazepane, 9) 1,3,6- trimethylpyrano[2,3-d ]imidazole-2,5(1H,3H)-dione, and 10) shanzhiol. The extract also exhibited an antibacterial activity against six bacterial strains: Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Salmonella enteritidis, as determined via disk diffusion agar method.
keywords:

Rothmannia wittii, extract, phytochemical composition, antibacterial activity, LC-MS

 
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