Production of an active human AID enzyme in a bacterial system

Lucyna Budzko; Paulina Jackowiak; Marek Figlerowicz

doi:10.5114/bta.2016.62360

eISSN: 2353-9461
ISSN: 0860-7796

BioTechnologia

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3/2016
vol. 97

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abstract:

SHORT COMMUNICATIONS

Production of an active human AID enzyme in a bacterial system

Lucyna Budzko

¹

,

Paulina Jackowiak

¹

,

Marek Figlerowicz

^{1, 2}

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland

Institute of Computing Science, Poznan University of Technology, Poznań, Poland

vol. 97(3) C pp. 235-239 C 2016

DOI: https://doi.org/10.5114/bta.2016.62360

Online publish date: 2016/11/02

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The aim of our invention is to characterize a modified gene of human activation-induced cytidine deaminase (hAID ), a method of modification of hAID gene, a composition showing hAID activity, and a method of preparation of such composition in a bacterial system. In particular, the invention concerns a modified hAID gene for the production of an active enzyme in a bacterial system as well as use of such composition for the analyzes of DNA/RNA amination/deamination, and/or methylation/demethylation.

Production of an active human AID enzyme in a bacterial system

Lucyna Budzko 1 , Paulina Jackowiak 1 , Marek Figlerowicz 1, 2

Lucyna Budzko

¹

,

Paulina Jackowiak

¹

,

Marek Figlerowicz

^{1, 2}