eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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1/2019
vol. 100
 
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abstract:
RESEARCH PAPERS

Production of indole acetic acid by Kocuria rosea VB1 and Arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates

Arun Karnwal

BioTechnologia vol. 100 (1) C pp. 29–35 C 2019
Online publish date: 2019/03/27
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Phytohormones play a very important role in enhancing plant growth by direct or indirect mechanisms involving plant-microbe interactions. Indole acetic acid (IAA) is one of the key phytohormones that directly enhance plant development. Kocuria rosea VB1 (GenBank ID: KY608093.1) and Arthrobacter luteolus VB2 (GenBank ID: KY608094.1) from polluted industrial water samples were characterized through a biochemical assay, 16S rDNA sequencing, and for promoting plant growth abilities. IAA production by VB1 and VB2 was tested in pure culture conditions supplemented with various L-tryptophan (Trp) concentrations (0, 50, 100, 200, and 500 μg • ml-1). A significant difference in indole production by VB2 and VB1 inoculant at various L-Trp concentrations has been observed. VB1 has been reported to produce increased amounts of indole from 0.33 μg • ml-1 to 18.16 μg • ml-1, while the increase in indole production was from 0.63 μg • ml-1 to 9.22 μg • ml-1 for VB2 for various L-Trp concentrations. The VB1 strain produced 85.07 ng • ml-1 and 123.7 ng • ml-1 IAA, whereas the VB2 strain produced 70.3 ng • ml-1 and 78.4 ng • ml-1 IAA, respectively at 200 and 500 μg • ml-1 Trp concentrations. The growth pouch experiments with maize root exudates also showed a positive effect for both bacterial inoculants tested on IAA biosynthesis in comparison to non-inoculated seeds. Inoculation of maize seeds with VB2 and VB1 bacteria gave a significantly higher level of IAA production in comparison to non-inoculated seeds. Current study outcomes show the beneficial aspects of plant growth regulators produced by free-living bacteria which could play a significant role in plant growth promotion.
keywords:

16s rDNA sequencing, phytohormones, plant growth promotion, rhizosphere, waste water

 
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