RESEARCH PAPER
Wild type root cultures of Scutellaria barbata

Scutellaria barbata wild type root cultures were grown on liquid media: Gamborg (B5) and Schenk-Hildebrandt

(SH) supplemented with (1 mg/l) indole-3-butyric acid (IBA). For each culture, growth rate and secondary metabolites

production were determined. It was observed that in the metabolic profiles, the dominating compounds were

flavone glycosides – wogonoside, baicalin and phenylethanoid glycoside acteoside. Aglycones namely wogonin,

baicalein and chrysin – were present at low concentrations. On B5 medium, the average productivity of plant material

counted as the sum of flavones was higher (ca. 1300 mg/l) than on SH medium (ca. 999 mg/l). In order to

remove the compounds that cause browning of the medium and inhibit culture growth, especially on B5 IBA 1.0

medium, a number of experiments with the addition of cork pieces, citric acid and Amberlite XAD-4 and XAD-7

resins into the culture media were performed. The best growth of the root culture was achieved on B5 medium

supplemented with – 1.0 mg/l IBA along with the addition of 7 mg/ml of cork and with 10 mg/ml of Amberlite

XAD-4 resin, although concentrations of the analyzed metabolites were lowers than in the control group.