eISSN: 2353-9461
ISSN: 0860-7796
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
vol. 104

Cytokine mediated immuno-regulatory activity of Lactobacillus species in carrageenan-induced acute inflammatory model

Babayemi O. Oladejo
Covenant F. Adeboboye
Precious I. Adiji
Tinuola T. Adebolu

  1. Department of Microbiology, Federal University of Technology, Akure, Nigeria
BioTechnologia vol. 104(1) ∙ pp. 53–63 ∙ 2023
Online publish date: 2023/03/27
View full text Get citation
PlumX metrics:
Using paw edema acute inflammatory model induced by carrageenan (1%) in Wistar rats, the immunoregulatory action of Lactobacillus sp., isolated from two locally fermented food products in Nigeria: Nunu (a yogurt-like milk product) and Ogi (guinea corn slurry), was investigated. The rats were distributed into seven groups (A–G). Rats in group A did not receive any therapy or carrageenan inflammation, whereas those in group B received a car¬ra¬ge¬enan injection only. Groups C–F were orally administered with lactic acid bacteria (LAB) strains (5 × 107 CFU/ml), where¬as group G received diclofenac sodium (150 mg/kg body weight) following the administration of carrageenan. At regular intervals, paw thickness (mm) was measured. Microscopy was used to count the number of leukocytes; myeloperoxidase (MPO) activity was used to measure the neutrophil accumulation in the paw tissue; and rat serum samples were subjected to ELISA to identify cytokine assays for C-reactive protein (CR-P), interleukin-10 (IL-10), and transforming growth factor-β (TGF-β). All of the LAB-treated groups showed a statistically significant decrease in paw thickness, and their neutrophil and monocyte infiltration was significantly affected. Compared with the control groups, oral administration with LAB significantly suppressed the MPO activity. Lactobacillus fermentum NBRC showed the most significant upregulation of serum levels of IL-10 and TGF-β though serum levels of CR-P were downregulated. Lactobacillus pentosus increased the production of TGF-β, with no significant effect on the production of IL-10. This study presents the role of Lactobacillus sp. in regulating inflammation by modifying the production of anti-inflammatory cytokines IL-10 and TGF-β.

carrageenan, cytokines, inflammation, lactic acid bacteria, edema

Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here