eISSN: 2353-9461
ISSN: 0860-7796
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
vol. 104

Exploring the potential of Crotalaria juncea flower extracts as a source of antioxidants, antimicrobials, and cytoprotective agents for biomedical applications

Pawika Mahasawat
Sawai Boukaew
Poonsuk Prasertsan

Programme in Biotechnology, Faculty of Science and Technology, Songkhla Rajabhat University, Songkhla, Thailand
College of Innovation and Management, Songkhla Rajabhat University, Songkhla, Thailand
Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University, Hatyai, Thailand
BioTechnologia vol. 104(4) ∙ pp. 359–370 ∙ 2023
Online publish date: 2023/12/21
View full text Get citation
PlumX metrics:
Plants provide an unlimited source of bioactive compounds, possessing tremendous applications in the pharmaceutical industry. In the search for sources of antioxidants and antimicrobial agents against human pathogens, ethanol extracts of Crotalaria juncea flowers (CJ flower extract) were evaluated. The highest total phenolic (5.65 μg GAE/ml) and flavonoid (0.43 μg QE/ml) contents were observed in the 100 μg/ml CJ flower extract. To assess antioxidant activity, three in vitro antioxidant tests were employed: DPPH radical-scavenging, ABTS+ radical-scavenging, and hydroxyl radical-scavenging assay. The CJ flower extract demonstrated significant (P < 0.05) antioxidant activity, dependent on the percentage of solvent extraction and the specific assays utilized. The highest antioxidant activity was obtained with 100% ethanol extraction and using the hydroxyl radical-sca¬venging assay (56.63%). Antimicrobial activity was assessed against six human pathogens, including the fungi Microsporum gypseum and five Gram-positive bacteria (Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, and Streptococcus mutans), as well as one Gram-negative bacterium (Escherichia coli). The CJ flower extract inhibited the growth of both fungal and bacterial pathogens. The cytotoxicity of the CJ flower extract was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the highest concentration of the extract (100 μg/ml) did not affect L929 cell viability. More¬over, the CJ flower extract demonstrated the ability to suppress H2O2-induced toxicity in L929 cells. Overall, the CJ flower extract has potential as an alternative source for exploring new antioxidants, antimicrobial agents, and cytoprotectants that could prove valuable for biomedical applications.

antioxidant activity, antimicrobial activity, anti H2O2-induced cytotoxicity, Crotalaria juncea Linn, flower extract

Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here