SHORT COMMUNICATION
Hyp-1 protein from St John’s wort as a PR-10 protein
 
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Publication date: 2014-10-23
 
 
BioTechnologia 2013;94(1):47-50
 
ABSTRACT
PR-10 proteins form a large subclass of plant pathogenesis-related proteins that are expressed in response to harmful
environmental factors in a wide range of species. Although their function is still not clear, structural data suggest
that their characteristic internal hydrophobic cavity can bind relevant plant small-molecule mediators. Hyp-1 from
St John's wort (Hypericum perforatum ), initially proposed as a catalyst for the biosynthesis of hypericin, was
eventually shown to share sequence similarity and a folding pattern with PR-10 proteins. The crystal structure of
Hyp-1 in complex with fluorescent probe ANS reveals three distinct and separated binding sites that are unique
among PR-10 proteins. The structure can provide guidance in our quest for the true physiological ligands of Hyp-1.
REFERENCES (8)
1.

Bais H., Vepachedu R., Lawrence C., Stermitz F., Vivanco J. (2003) J. Biol. Chem. 278: 32413-32422.
 
2.

Biesiadka J., Bujacz G., Sikorski M., Jaskolski M. (2002) J. Mol. Biol. 319: 1223-1234.
Fernandes H., Michalska K., Sikorski M., Jaskolski M. (2013) FEBS J. 280: 1169-1199.
Fernandes H., Konieczna M., Kołodziejczyk R., Bujacz G., Sikorski M., Jaskolski M. (2008) Acta Cryst. F64: 405-408.
 
3.

Fernandes H., Pasternak O., Bujacz G., Bujacz A., Sikorski M., Jaskolski M. (2008) J. Mol. Biol. 378: 1040-1051.
 
4.

Kofler S., Asam C., Eckhard U., Wallner M., Ferreira F., Brandstetter H. (2012) J. Mol. Biol. 422: 109-123.
 
5.

Kosuth J., Jaskolski M., Cellarova E. (2013) Plant Cell Tiss. Org., in press.
 
6.
Kundu B., Guptasarma P. (2002) Biochem. Biophys. Res. Commun. 293: 572-577.
 
7.

Michalska K., Fernandes H., Sikorski M., Jaskolski M. (2010) J. Struct. Biol. 169: 161-171. Pasternak O., Bujacz G., Fujimoto Y., Hashimoto Y., Jelen F., Otlewski J., Sikorski M.,
Jaskolski M. (2006) Plant Cell. 18: 2622-2634.
 
8.

Umezawa T., Nakashima K., Miyakawa T., Kuromori T., Tanokura M., Shinozaki K., Yamaguchi-Shinozaki K. (2010) Plant Cell Physiol. 51: 1821-1839.
 
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