RESEARCH PAPER
In vitro screening of angiotensin converting enzyme and hydroxymethylglutaryl-coenzyme A reductase inhibitory activities in lactic acid bacteria and yeasts isolated from fermented sorghum gruels
1
Department of Microbiology, Osun State University, Osogbo, Osun State, Nigeria
2
Food Microbiology and Biotechnology Unit, Department of Microbiology, University of Ibadan, Oyo State, Nigeria
3
Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America
4
Phytochemistry and Toxicology Unit, Royal Scientific Research Institute, Osun State, Nigeria
Submission date: 2024-07-29
Final revision date: 2024-09-07
Acceptance date: 2025-05-22
KEYWORDS
TOPICS
ABSTRACT
Background:
3-hydroxyl-3-methylglutaryl-coenzyme A (HMG-CoA) reductase and angiotensin converting enzyme (ACE) are implicated in the pathogenesis of hyperlipidemia and hypertension, which are oxidative-stress linked conditions of public health importance. The adverse effects associated with standard clinical drugs used to inhibit these enzymes have prompted the search for alternative sources. This study was designed to investigate the in vitro inhibitory activities of lactic acid bacteria (LAB) and yeasts isolated from fermented sorghum gruels.
Material and methods:
LAB and yeast isolates were obtained and characterized using standard methods. The HMG-CoA reductase and ACE inhibitory activities of the microbial isolates were evaluated using established protocols.
Results:
Screening of LAB for HMG-CoA reductase and ACE inhibitory activities revealed that at concentrations (mg/ml) of 6, 12, 24, and 48, Lactobacillus pentosus WSL5 exhibited the highest %HMG-CoA reductase inhibition of 3.21, 6.42, 9.17, and 12.84, with corresponding ACE inhibitory activities of 6.38, 13.17, 18.13, and 23.47, respectively. At concentrations (mg/ml) of 1, 2, 4, and 8, the yeast isolates Trichomonascus ciferri RSY53 demonstrated %HMG-CoA reductase inhibition of 7.71, 11.47, 14.68, and 16.97, with corresponding ACE inhibitory activities of 11.83, 20.91, 34.73, and 48.28, respectively. Furthermore, L. pentosus WSL5 recorded the lowest HMG-CoA reductase half-maximal inhibitory concentration (IC50) of 219.72 µg/ml and ACE IC50 of 116.22 µg/ml, while T. ciferri RSY53 had even lower IC50 values of 29.55 µg/ml for HMG-CoA reductase and 7.03 µg/ml for ACE inhibition compared to the controls.
Conclusions:
L. pentosus WSL5 and T. ciferri RSY53 can be considered potential starter cultures for the fermentation of functional foods aimed at supporting cardiovascular health.
3-hydroxyl-3-methylglutaryl-coenzyme A (HMG-CoA) reductase and angiotensin converting enzyme (ACE) are implicated in the pathogenesis of hyperlipidemia and hypertension, which are oxidative-stress linked conditions of public health importance. The adverse effects associated with standard clinical drugs used to inhibit these enzymes have prompted the search for alternative sources. This study was designed to investigate the in vitro inhibitory activities of lactic acid bacteria (LAB) and yeasts isolated from fermented sorghum gruels.
Material and methods:
LAB and yeast isolates were obtained and characterized using standard methods. The HMG-CoA reductase and ACE inhibitory activities of the microbial isolates were evaluated using established protocols.
Results:
Screening of LAB for HMG-CoA reductase and ACE inhibitory activities revealed that at concentrations (mg/ml) of 6, 12, 24, and 48, Lactobacillus pentosus WSL5 exhibited the highest %HMG-CoA reductase inhibition of 3.21, 6.42, 9.17, and 12.84, with corresponding ACE inhibitory activities of 6.38, 13.17, 18.13, and 23.47, respectively. At concentrations (mg/ml) of 1, 2, 4, and 8, the yeast isolates Trichomonascus ciferri RSY53 demonstrated %HMG-CoA reductase inhibition of 7.71, 11.47, 14.68, and 16.97, with corresponding ACE inhibitory activities of 11.83, 20.91, 34.73, and 48.28, respectively. Furthermore, L. pentosus WSL5 recorded the lowest HMG-CoA reductase half-maximal inhibitory concentration (IC50) of 219.72 µg/ml and ACE IC50 of 116.22 µg/ml, while T. ciferri RSY53 had even lower IC50 values of 29.55 µg/ml for HMG-CoA reductase and 7.03 µg/ml for ACE inhibition compared to the controls.
Conclusions:
L. pentosus WSL5 and T. ciferri RSY53 can be considered potential starter cultures for the fermentation of functional foods aimed at supporting cardiovascular health.
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