RESEARCH PAPER
Interactive effects of plant growth regulators and explants on direct shoot regeneration of Viola odorata
1
Department of Plant Tissue Culture, Branch of Central Region of Iran,
Agricultural Biotechnology Research Institute of Iran (ABRII), Isfahan, Najafabad, Iran
2
Department of Agricultural Biotechnology, Payam Noor University of Isfahan, Isfahan, Iran
Submission date: 2016-01-02
Final revision date: 2016-02-21
Acceptance date: 2016-02-24
Publication date: 2016-04-20
BioTechnologia 2016;97(1):33-39
KEYWORDS
TOPICS
ABSTRACT
The aim of the study was to develop a protocol for in vitro shoot regeneration based on a solid Murashige and Skoog (MS) basal medium supplemented with various concentrations of plant growth regulators (PGRs) like Indole-3-butyric acid (IBA), kinetin (KIN) or benzylaminopurine (BAP) using leaf, petiole, root, and crown disk segments of a valuable medicinal plant Viola odorata. The best percentage of direct shoot regeneration (67.50%) and the maximum number of micro-shoots (9.50) were obtained with crown explants on MS medium containing IBA (0.5 mgA l!1) combined with BAP (3 or 4 mg A l!1). The best elongation (5.25 cm) of micro-shoots was observed in crown explants on a medium containing 1 mg A l!1 gibberellic acid (GA3) followed by GA3 at 0.5 mg A l!1 and similar explants. Irrespective of the concentrations of GA3, the minimum length of shoots was observed in leaf explants. A half-strength MS medium containing activated charcoal and supplemented with 1.5 mg A l!1 IBA resulted in the best rooting (78%) of elongated shoots. All acclimatized plantlets survived and showed a normal growth. The study reports a simple and applicable protocol for micropropagation of V. odorata through direct organogenesis.
REFERENCES (28)
1.
Ajithkumar D., Seeni S. (1998) Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L) Corr A Medicinal Tree. Plant Cell Rep. 17: 422- 426.
2.
Al-Khateeb A. (2008) Comparison effects of sucrose and date palm syrup on somatic embryogenesis of date palm (Phoenix dactylifera L.). Am. J. Biochem. Biotech. 4: 19-23.
3.
Azad M.A.K., Amin M.N., Begum F. (1999) In vitro rapid regeneration of plantlets from cotyledon explant of Adhatoda vasica Nees. Plant Tiss. Cult. 9: 121-126.
4.
Azad M.A.K., Yokota S., Ohkubo T., Andoh Y., Yahara S., Yoshizawa N. (2005) In vitro regeneration of the medicinal woody plant Phellodendron amurense Rupr. Through excised leaves. Plant Cell Tiss. Org. 83: 43-50.
5.
Barekat T., Othroshy M., Samsamzadeh B., Sadrarhami A., Mokhtari A. (2013) A novel approach for breaking seed dormancy and germination in Viola odorata (A medicinal plant). J. Nove. Appl. Sci. 2: 513-516.
6.
Bekheet S.A., Saker M.M. (1998) In vitro propagation of Egyptian Date Palm: II. Direct and indirect shoot proliferation from shoot tip explants of Phoenix dactylifera L. cv Zaghlool. [In:] Proceedings first international conference on date palm. Ed. Al Ain, UAE: 150-157.
7.
Bogaert I., Van Cauter S., Werbrouck S.P.O., Dolezal K. (2006) New aromatic cytokinins can make the diff erence. [In:] V International Symposium on In Vitro Culture and Horticultural Breeding 725, pp. 265-270.
8.
Ebrahimzadeh M.A., Nabavi S.f., Nabavi S.M., Slami B.E. (2010) Antioxidant and free radical scavenging activity of H. officinalis L. var. angustifolius, V. odorata, B. hyrcana and C. speciosum. Pak. J. Pharm. Sci. 23: 29-34.
9.
George E.F., Hall M.A., De Klerk G.J. (2008) Micropropagation: uses and methods. [In:] Plant propagation by tissue culture. Netherlands, Springer: 29-64.
10.
Ghanti K.R.B., Venugopal F.T.Z., Jabeen C.P., Rao S. (2004) Rapid regeneration of Mentha piperita L. from shoot tip and nodal eexplants. Indian J. Biotech. 3: 594-598.
11.
Gray W.M. (2004) Hormonal regulation of plant growth and development. PLoS Bio. 2: 1270-1273.
12.
Gustafson K.R., McKee T.C., Bokesch H.R. (2004) Anti-HIV cyclotides. Curr. Protein Pept. Sci. 5: 331-340.
13.
Haghighat Afshar M., Jafarkhani M., Khalighi A., Habashi A., Mohamadi A. (2011) Direct shoot regeneration on three cultivars of rosa hybrid using five explant types and different hormone concentrations. Amer.-Eurasian J. Agric. Environ. Sci. 10: 962-967.
14.
Ireland D.C., Colgrave M.L., Craik D.G. (2006) A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability. Biochem. J. 400:1-12.
15.
Kaloo A.Z.R., Akhtar Z., Wafai B.A. (2012) Effect of growth regulators on the in vitro multiplication of Viola odorata. Int. J. Med. Plant Res. 4: 187-189.
16.
Kalyani B.G., Rao S. (2014) Effect of hormones on direct shoot regeneration in leaf explants of tomato. Int. J. Res. Biotech. Biochem. 4: 20-22.
17.
Li J.T., Deng D.M., Peng G.T., Deng J.C., Zhang J., Liao B. (2010) Successful micropropagation of the cadmium hyper accumulator Viola baoshanensis (Violaceae). Int. J. Phytoremediation. 12: 160-210.
18.
Lindholm P., Goransson U., Johansson S., Claeson P., Gulibo J., Larsson R., Bohlin L., Backlund A. (2002) Cyclotides a novel type of cytotoxic agents. Mol. Cancer Ther. 1: 365- 369.
19.
Lord A.M.M. (1983) Comparative Flower Development in the Cleistogamous Species Viola odorata. I. A Growth Rate Study. Amer. J. Bot. 1: 1556-1563.
20.
Martin K.P. (2002) Rapid propagation of Holostemma adakodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Rep. 21: 112-117.
21.
Mokhtari A. (2016) Medicinal Plants: Sweet violet (Viola odorata), Description, Medicinal uses and Micropropagation. Saarbrucken, Deutschland/Germany: LAP LAMBERT Academic Publishing.
22.
Mokhtari A., Otroshy O., Barekat T. (2015) Plant regeneration through callus induction on medicinal herb Viola odorata -role of plant growth regulators and explants. Agricult. Forest. 61: 3-16.
23.
Nandagopal S., Kumari R.B.D. (2007) Effectiveness of auxin induced in vitro root culture in chicory. J. Cen. Europ. Agri. 8: 73-79.
24.
Taiz L., Zeiger E. (1998) Plant Physiology, 2nd ed. Sinauer Associates, Sunderland, MA, USA.
25.
Tiwari K.N., Sharma N.C., Tiwari V., Singh B.D. (2000) Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tiss. Org. Cult. 63: 179-185.
26.
Udayakumar R., Choi C.W., Kim K.T., Kim S.C., Kasthurirengan S., Mariashibu T.S., Sahaya Rayan J.J., Ganapathi A. (2013) In vitro plant regeneration from epicotyl explant of Withania somnifera (L.) Dunal. J. Med. Plants Res. 7: 43-52.
27.
Verma S.K., Yucesan B.B., Sahin G., Gurel S., Gurel E. (2011) Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. Turk. J. Bot. 35: 689-695.
28.
Vuylasteker C., Dewaele S., Rambour S. (1998) Auxin induced lateral root formation in chicory. Ann. Bot. 81: 449-454.
| eISSN: | 2353-9461 |
| ISSN: | 0860-7796 |
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