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vol. 105

Phytochemical composition, antioxidant, antibacterial, and enzyme inhibitory activities of organic extracts from flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry

Doan Thien Thanh
1, 2
Vo Kieu Oanh
Hoang Chinh Nguyen
Luong Thi My Ngan
Tran Trung Hieu

  1. Faculty of Biology and Biotechnology, VNUHCM-University of Science, Ho Chi Minh City, Vietnam
  2. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam
  3. School of Life and Environmental Sciences, Deakin University, Geelong, Australia
BioTechnologia vol. 105(2) ∙ pp. 137–147 ∙ 2024
Online publish date: 2024/06/25
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Cleistocalyx operculatus flower buds have been widely used in traditional medicine because of their rich content of bioactive constituents. In this study, we obtained seven solvent extracts from the flower buds and evaluated their total phenolic (TPC), flavonoid (TFC), tannin (TTC), triterpenoid saponin (TSC), and alkaloid (TAC) con­tents. We assessed antioxidant activities using the DPPH assay and also looked at antimicrobial and enzyme in­hibi­tory effects. The water extract possessed the highest TPC (328.9 mg GAE/g extract), followed by ethanol, me­thanol, and hexane extracts (85.4–101.5 mg GAE/g extract). Chloroform, butanol, ethyl acetate, and ethanol extracts had high TSCs (245.4–287.2 mg OAE/g extract). The hexane extract was richest in TTC and TFC (32.7 mg CE/g extract and 81.1 mg QE/g extract, respectively). Ethanol and methanol extracts exhibited the strongest antioxidant activities (IC50 values of 25.2 and 30.3 μg/ml, respectively), followed by the water extract (IC50 of 40.2 μg/ml). The hexane extract displayed the most growth-inhibitory activity against Helicobacter pylori ATCC51932 and ATCC43504 strains and Salmonella enterica serovar Typhimurium ATCC13311 (MIC values of 0.06, 0.13, and 0.4 mg/ml, respectively). Moreover, the hexane extract revealed the strongest inhibition of H. pylori urease acti­vity (IC50 of 4.51 μg/ml), whereas the water and methanol extracts had potent inhibitory effects on α-glucosidase activity (IC50 values of 9.9 and 15.1 μg/ml, respectively). These flower bud extracts could be used for health pro­tection, especially in preventing bacterial infections and inhibiting enzymes associated with various human dis­eases. Further investigation into the application of C. operculatus flower buds in the food and pharmaceutical industries is necessary.

Cleistocalyx operculatus, flower bud, phytochemical composition, antioxidant activity, antibacterial activity, enzyme inhibition

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