eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
Newsletter
Enter your email:
 
Search
Editorial Policies
Sarajevo Declaration on Integrity and Visibility of Scholarly Publications
2/2023
vol. 104
 
Share:
Send email
Share:
abstract:
RESEARCH PAPERS

Potent antifungal properties of gallic acid in Sarcochlamys pulcherrima against Candida auris

Nahid Akhtar
1
,
M. Amin-ul Mannan
1, 2
,
Deeksha Pandey
3
,
Amon Sarkar
1
,
Himanshi Sharma
1
,
Manish Kumar
3
,
Anup Ghosh
4

  1. Department of Molecular Biology and Genetic Engineering, School of Bioengineering and Biosciences, Lovely Professional University, Jalandhar-Delhi, India
  2. Division of Infectious Disease, The Lundquist Institute for Biomedical Innovation at Harbor, University of California, Los Angeles (UCLA) Medical Center, Los Angeles, CA, USA
  3. Department of Biophysics, University of Delhi-South Campus, New Delhi, India
  4. Department of Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
BioTechnologia vol. 104(2) ∙ pp. 105–119 ∙ 2023
DOI: https://doi.org/10.5114/bta.2023.127202
Online publish date: 2023/06/26
View full text Get citation
 
PlumX metrics:
Candida auris is a major public health concern due to its high transmission and mortality rates, as well as the emergence of pan-resistant strains. This study aimed to identify an antifungal compound from Sarcochlamys pulcherrima, an ethnomedicinal plant, that can inhibit the growth of C. auris. Methanol and ethyl acetate extracts of the plant were obtained, and high-performance thin-layer chromatography (HPTLC) analysis was conducted to identify the major compounds in the extracts. The major compound detected by HPTLC was subjected to in vitro antifungal activity testing, and its antifungal mechanism was determined. The plant extracts inhibited the growth of both C. auris and Candida albicans. HPTLC analysis revealed the presence of gallic acid in the leaf extract. Furthermore, the in vitro antifungal assay showed that gallic acid inhibited the growth of different C. auris strains. In silico studies indicated that gallic acid can bind to the active sites of carbonic anhydrase (CA) proteins in both C. auris and C. albicans, affecting their catalytic activities. Compounds that target virulent proteins such as CA can aid in the reduction of drug-resistant fungi and the development of novel antifungal compounds with unique modes of action. However, additional in vivo and clinical studies are required to conclusively determine gallic acid’s antifungal properties. Gallic acid derivatives may be developed in the future to possess more potent antifungal properties and target various pathogenic fungi.
keywords:

Sarcochlamys pulcherrima, antifungal, gallic acid, Candia auris, molecular docking, carbonic anhydrase

  
Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here
Hosted by Termedia sp. z o.o.