SHORT COMMUNICATION
Protuberance-mediated high frequency of adventitious
shoot regeneration from sweetpotato leaf explants
[Ipomoea batatas (L.) Lam.]
Publication date: 2014-10-23
BioTechnologia 2013;94(4):445-450
KEYWORDS
ABSTRACT
In this report, a novel, reproducible and efficient protocol for protuberance-mediated adventitious shoot regeneration
has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified
Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron
(TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented
with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium
containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants.
It appeared that the frequency of protuberance development depended on the concentration of TDZ. However,
this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious
shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin
of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS
medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully
established in a greenhouse.
has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified
Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron
(TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented
with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium
containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants.
It appeared that the frequency of protuberance development depended on the concentration of TDZ. However,
this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious
shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin
of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS
medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully
established in a greenhouse.
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| ISSN: | 0860-7796 |
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