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Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [Ipomoea batatas (L.) Lam.]
 
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Publication date: 2014-10-23
 
 
BioTechnologia 2013;94(4):445-450
 
KEYWORDS
ABSTRACT
In this report, a novel, reproducible and efficient protocol for protuberance-mediated adventitious shoot regeneration
has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified
Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron
(TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented
with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium
containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants.
It appeared that the frequency of protuberance development depended on the concentration of TDZ. However,
this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious
shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin
of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS
medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully
established in a greenhouse.
REFERENCES (34)
1.

Annapurna D., Rathore T.S. (2010) Direct adventitious shoot induction and plant regeneration of Embelia ribes Burm F. Plant Cell Tissue Organ. Cult. 101: 269-277. Artsaenko O., Kettig B., Fiedler U., Conrad U., Düring K. (1998) Potato tubers as a biofactory for recombinant antibodies. Mol. Breed. 4: 313-319.
 
2.

Berberich T., Takagi T., Miyazaki A., Otani M., Shimada T., Kusano T. (2005) Production of mouse adiponectin, an antidiabetic protein, in transgenic sweetpotato plants. J. Plant Physiol. 162: 1169-1176.
 
3.

Bieniek M.E., Harrell R.C., Cantliffe D.J. (1995) Enhancement of somatic embryogenesis of Ipomoea batatas in solid cultures and production of mature somatic embryos in liquid cultures for application to a bioreactor production system. Plant Cell Tiss. Org. Cult. 41: 1-8.
Chée R.P., Schultheis J.R., Cantliffe D.J. (1990) Plant recovery from sweetpotato somatic embryos. HortSci. 25: 795-797.
 
4.

Corredoira E., Ballester A., Vieitez A.M. (2008) Thidiazuroninduced high-frequency plant regeneration from leaf explants of Paulownia tomentosa mature trees. Plant Cell Tiss. Org. Cult. 95: 197-208.
 
5.

Dai W., Su Y., Castillo C., Beslot O. (2011) Plant regeneration from in vitro leaf tissues of Viburnum dentatum L. Plant Cell Tiss. Org. Cult. 104: 257-262.
 
6.

Debnath S.C. (2005) A two-step procedure for adventitious shoot regeneration from in vitro-derived lingonberry leaves: shoot induction with TDZ and shoot elongation using zeatin. HortSci. 40: 189-192. Debnath S.C. (2009) A two-step procedure for adventitious shoot regeneration on excised leaves of lowbush blueberry. In Vitro Cell Dev. Biol. Plant 45: 122-128.
 
7.

Desamero N.V., Rhodes B.B., Decoteau D.R., Bridges W.C. (1994) Picolinic acid-induced direct somatic embryogenesis in sweetpotato. Plant Cell Tiss. Org. Cult. 37: 103-111.
 
8.

Dessai A.P., Gosukonda R.M., Blay E., Dumenyo C.K., Medina-Bolivar F., Prakash C.S. (1995) Plant regeneration of sweetpotato (Ipomoea batatas L.) from leaf explants in vitro using a two-stage protocol. Sci. Horti. 62: 217-224.
 
9.

Dhir S.K., Oglesby J., Bhagsari A.S. (1998) Plant regeneration via somatic embryogenesis, and transient gene expression in sweetpotato protoplasts. Plant Cell Rep. 17: 665-669.
 
10.

Faisal M., Ahmad N., Anis M. (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss. Org. Cult. 80: 187-190.
 
11.

Fowke L.C., Rennie P.J. (1996) Botanical microtechnique for plant cultures. In: Plant Cell, Tissue and Organ Culture, Fundamental methods, eds. Gamborg O.L., Phillips G.C., Narosa Publishing House, New Delhi, pp. 217-228.
 
12.

Gama M.I.C.S., Leite Jr. R.P., Cordeiro A.R., Cantliffe D.J. (1996) Transgenic sweet potato plants obtained by Agrobacterium tumefaciens-mediated transformation. Plant Cell Tiss. Org. Cult. 46: 237-244.
 
13.

Gosukonda R.M., Dessai A.P., Blay E., Prakash C.S., Peterson C.M. (1995) Thidiazuron-induced adventitious shoot re-generation of sweetpotato (Ipomoea batatas). In Vitro Cell Dev. Biol. Plant 31: 65-71.
 
14.

He D.G., Yang Y.M., Bertram J., Scott K.J. (1990) The histological development of the regenerative tissue derived from cultured immature embryos of wheat (Triticum aestivum L.). Plant Sci. 68: 103-111.
 
15.

He S., Han Y., Wang Y., Zhai H., Liu Q. (2009) In vitro selection and identification of sweetpotato (Ipomoea batatas (L.) Lam.) plants tolerant to NaCL. Plant Cell Tiss. Org. Cult. 96: 69-74.
 
16.

Hervé P., Jauneau A., Pâques M., Marien J.-N., Boudet A.M., Teulières C. (2001) A procedure for shoot organogenesis in vitro from leaves and nodes of an elite Eucalyptus gunnii clone: comparative histology. Plant Sci. 161: 645-653.
 
17.

Ledbetter D.I., Preece J.E. (2004) Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. leaf explants. Sci Horti. 101: 121-126.
 
18.

Liu C., Callow P., Rowland L.J., Hancock J.F., Song G.-Q. (2010) Adventitious shoot regeneration from leaf explants of southern high bush blueberry cultivars. Plant Cell Tiss. Org. Cult. 103: 137-144.
 
19.

Liu J.R., Cantliffe D.J. (1984) Somatic embryogenesis and plant regeneration in tissue cultures of sweetpotato (Ipomoea batatas Poir.). Plant Cell Rep. 3: 112-115.
 
20.

Ma C., Ye X., Chen Y., Feng J., Shang X., Li J., Wu Y., Hu J. (2012) Anatomical observations of adventitious bud regeneration from leaf explants of Ziziphus jujube Mill. ‘Huizao’. Hort. Environ. Biotechnol. 53: 316-319.
 
21.

Mithila J., Hall J.C., Victor J.M.R., Saxena P.K. (2003) Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.). Plant Cell Rep. 21: 408-414.
 
22.

Mok M.C., Mok D.W.S., Armstrong D.J., Shudo K., Isogai Y., Okamoto T. (1982) Cytokinin activity of N-phenyl-N-1,2,3- thidiazol-5-ylurea (thidiazuron). Phytochemistry 21: 1509- 1511.
 
23.

Moran R., Garca R., Lopez A., Zaldua Z., Mena J., Garca M., Armas R., Somonte D.,

Rodrguez J., Gomez M., Pimentel E. (1998) Transgenic sweet potato plants carrying the delta-endotoxin gene from Bacillus thuringiensis var. tenebrionis. Plant Sci. 139: 175-184.

MroginskiE., Rey H.Y., GonzalezA.M., MroginskiL.A. (2004) Thidiazuron promotes in vitro plant regeneration of Arachis correntina (Leguminosae) via Organogenesis. J. Plant Growth Reg. 23: 129-134.
 
24.

Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473-497.
 
25.

Murthy B.N.S., Murch S.J., Saxena P.K. (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev. Biol. 34: 267-275. Newell C.A., Lowe J.M.,.
 
26.

Merryweather A., Rooke L.M., Hamilton W.D.O. (1995) Transformation of sweetpotato (Ipomoea batatas (L.) Lam.) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin. Plant Sci. 107: 215-227.
 
27.

Nitnaware K.M., Naik D.G., Nikam T.D. (2011) Thidiazuroninduced shoot organogenesis and production of hepatoprotective lignan phyllanthin and hypophyllanthin in Phyllanthus amarus. Plant Cell Tiss. Org. Cult. 104: 101-110.
 
28.

Okada Y., Saito A., Nishiguchi M., Kimura T., Mori M., Hanada K., Sakai J., Miyazaki C., Matsuda Y., Murata T. (2001) Virus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)] expressing the coat protein gene of sweetpotato feathery mottle virus. Theor. Appl. Genet. 103: 743- 751.
 
29.

Pinto A.P.C., Monteiro-Hara A.C.B.A., Stipp L.C.L., Mendes B.M.J. (2010) In vitro organogenesis of Passiflora alata. In Vitro Cell Dev. Biol. Plant. 46: 28-33.
 
30.

Santa-Maria M., Pecota K.V., Yencho C.G., Allen G., Sosinski B. (2009) Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes. Plant Cell Tiss. Org. Cult. 97: 109-117.
 
31.

Sihachakr D., Haïcour R., Alves J.M.C., Umboh I., Nzoghé D., Servaes A., Ducreux G. (1997) Plant regeneration in sweetpotato (Ipomoea batatas L., Convolvulaceae). Euphytica 96: 143-152.
 
32.

Sujatha M., Makkar H.P.S., Becker K. (2005) Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Reg. 47: 83-90.
 
33.

Woolfe J.A. (1992) Sweetpotato, an untapped food resource. Cambridge University Press,
New York. Yu B., Zhai H., Wang Y., Zang N., He S., Liu Q. (2007) Efficient Agrobacterium tumefaciens – mediated transformation using embryogenic suspension cultures in sweetpotato, Ipomoea batatas (L.) Lam. Plant Cell Tiss. Org. Cult. 90: 265-273.
 
34.

Zheng Q., Dessai A.P., Prakash C.S. (1996) Rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis. Plant Cell Rep. 15: 381-385.
 
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