Intense development of the transport sector and a rise in the prices of fossil fuels boost the demand for fuels from
alternative sources of energy, including biofuels. Thus, new energy-efficient technologies of fuel production from
renewable resources are being developed. Accordingly, we attempted to create a technology of bioethanol production
by simultaneous enzymatic hydrolysis and fermentation of native corn starch. In our work we used commercially
available STARGEN 001 enzymatic preparation for native corn starch hydrolyzis, and yeast Saccharomyces
cerevisiae strain Red Star Ethanol Red for it’s fermentation. A major part of the research was on developing
a fermentation technology with repeated full recirculation of stillage liquid fraction. It has been shown that
a completely effluentless ethanol production with separation of stillage solid fraction and using the liquid fraction
to prepare the next portion of corn mash is possible. Thus the process can proceed for several months without
any effluent.