The present invention relates to an RNA oligomer, methods for regulating’a microRNA (miRNA) production process and RNA oligomers used as miRNA production process regulators. More precisely, the present invention relates to use of RNA oligomers disrupting a pre- iRNA structure as miRNA production process regulators. Oligomer interactions with a miRNA precursor (pre-miRNA) change the precursor’s secondary and tertiary structure. In consequence, the pre-miRNA is not specifically recognised and cleaved by the Dicer ribonuclease, and the specific miRNA is not produced
REFERENCES(19)
1.
Ambros V. (2003) MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing. Cell 113: 673-676.
Bernstein E., Caudy A.A., Hammond S.M., Hannon G.J. (2001) Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409: 363-366.
Han J., Lee Y., Yeom K.H., Nam J.W., Heo I., Rhee J.K., Sohn S.Y., Cho Y., Zhang B.T., Kim V.N. (2006) Molecular basis for the recognition of primary microRNAs by the Drosha- DGCR8 complex. Cell 125: 887-901.
Jackowiak P., Figlerowicz M., Kurzynska-Kokorniak A., Figlerowicz M. (2011) Mechanisms involved in the development of chronic hepatitis C as potential targets of antiviral therapy. Curr. Pharm. Biotechnol. 12: 1774-1780.
Kurzynska-Kokorniak A., Jackowiak P., Figlerowicz M., Figlerowicz M. (2009) Human- and virus-encoded microRNAs as potential targets of antiviral therapy. Mini Rev. Med. Chem. 9: 927-937.
Kurzynska-Kokorniak A., Koralewska N., Tyczewska A., Twardowski T., Figlerowicz M. (2013) A new short oligonucleotide- based strategy for the precursor-specific rRegulation of microRNA processing by Dicer. PLoS One 8: e77703.
Kurzynska-Kokorniak A., Koralewska N., Pokornowska M., Urbanowicz A., Tworak A., Mickiewicz A., Figlerowicz M. (2015) The many faces of Dicer: the complexity of the mechanisms regulating Dicer gene expression and enzyme activities. Nucl. Acids Res. 43: 4365-4380.
Provost P., Dishart D., Doucet J., Frendewey D., Samuelsson B., Radmark O. (2002) Ribonuclease activity and RNA binding of recombinant human Dicer. EMBO J. 21: 5864- 5874.
Tyczewska A., Kurzynska-Kokorniak A., Koralewska N., Szopa A., Kietrys A.M., Wrzesinski J., Twardowski T., Figlerowicz M. (2011) Selection of RNA oligonucleotides that can modulate human dicer activity in vitro. Nucl. Acid Ther. 21: 333-346.
Zhang H., Kolb F.A., Brondani V., Billy E., Filipowicz W. (2002) Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP. EMBO J. 21: 5875-5885.
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.