SHORT COMMUNICATION
The effect of growth regulators on the regeneration ability of flax (Linum usitatissimum L.) hypocotyl explants in in vitro culture
The effect of growth regulators on the regeneration ability of flax (Linum usitatissimum L.) hypocotyl explants in in vitro culture
Publication date: 2014-10-28
BioTechnologia 2012;93(2):135-138
KEYWORDS
ABSTRACT
The aim of the present study is to determine the influence of different concentrations of growth regulators on
the hypocotyl explants regeneration abilities of flax cultivars (Linum usitatissimum L.). Plant material used in
the study was obtained from two flax cultivars: Modran and Selena. Hypocotyl explants were obtained from 6-dayold
seedlings and were subsequently placed on basal MS (Murashige and Skoog 1962) medium and MS medium
supplemented with 6-benzylaminopurine (BAP) or naftalene acetic acid (NAA). Final evaluation of the callus,
the shoot and the root formation of the explants was performed 28 days after the experimental setup. The highest
regeneration effectiveness was observed on the media supplemented with 1 mg/l BAP.
the hypocotyl explants regeneration abilities of flax cultivars (Linum usitatissimum L.). Plant material used in
the study was obtained from two flax cultivars: Modran and Selena. Hypocotyl explants were obtained from 6-dayold
seedlings and were subsequently placed on basal MS (Murashige and Skoog 1962) medium and MS medium
supplemented with 6-benzylaminopurine (BAP) or naftalene acetic acid (NAA). Final evaluation of the callus,
the shoot and the root formation of the explants was performed 28 days after the experimental setup. The highest
regeneration effectiveness was observed on the media supplemented with 1 mg/l BAP.
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| eISSN: | 2353-9461 |
| ISSN: | 0860-7796 |
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