eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
2/2024
vol. 105
 
Share:
Share:
abstract:
RESEARCH PAPERS

Secondary metabolites, spectra characterization, and antioxidant correlation analysis of the polar and nonpolar extracts of Bryophyllum pinnatum (Lam) Oken

Chinyere E. Okafor
1
,
Ikechukwu K. Ijoma
2
,
Chiamaka A. Igboamalu
1
,
Chinaza E. Ezebalu
1
,
Chukwuemeka F. Eze
1
,
Jessica C. Osita-Chikeze
1
,
Chisom E. Uzor
1
,
Adaugo L. Ekwuekwe
1

  1. Department of Pure and Industrial Chemistry, Chukwuemeka Odumegwu Ojukwu University, Uli, Nigeria
  2. Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University, Awka, Nigeria
BioTechnologia vol. 105(2) ∙ pp. 121–136 ∙ 2024
Online publish date: 2024/06/25
View full text Get citation
 
PlumX metrics:
Oxidative stress-related pathologies have guided the scientific community into delving into natural product-based research on plant-based metabolites. Plant secondary metabolites serve as a valid alternative in managing oxidative stress-related pathologies. In this study, we present the secondary metabolite constituents of the polar extract (PE) and nonpolar extract (NPE) from the leaves of Bryophyllum pinnatum. These constituents were determined through qualitative and quantitative phytochemical screening. The functional groups and structures of these metabolites were determined based on FTIR and GC-MS experiments, respectively. Antioxidant and free radical scavenging (FRS) activities were determined using standard methods, including phosphomolybdenum, FRAP, DPPH, HRSA, and reducing power assays, with comparisons made to the ascorbic acid (AA) standard. Through Pearson correlation analysis, we estimated the relationship between antioxidant and FRS activities. The DPPH results revealed IC50s of 380.104 ± 0.001, 16.763 ± 0.001, and 7.684 ± 0.003 μg/mL for NPE, AA, and PE, respectively, indicating a trend of PE > AA > NPE. However, all other experiments showed a trend of AA > PE > NPE in antioxidant and FRS activities. These results showed the potential antioxidant and FRS properties of both PE and NPE. Additionally, the correlation analysis indicated a strong positive correlation between the antioxidant and FRS activities of PE and NPE. The research results suggest high antioxidant and FRS activities of PE and validate the use of B. pinnatum in managing free radical-related pathologies.
keywords:

spectrophotometry, correlation, ethnomedicine, phytochemical, absorbance

 
Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here