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RESEARCH PAPER
In vitro propagation of pascuita (Euphorbia leucocephala Lotsy): effects of different LED light colors on callus, shoots, and root induction
1
Universidad Autónoma Chapingo, Texcoco, Estado de México, Mexico
2
Colegio de Postgraduados, Texcoco, Estado de México, Mexico
Submission date: 2025-11-17
Final revision date: 2026-02-02
Acceptance date: 2026-03-30
Publication date: 2026-06-27
BioTechnologia 2026;107(2):111-120
KEYWORDS
TOPICS
ABSTRACT
Background:
The application of different light emitting diodes (LED) light colors in plant micropropagation is gaining prominence based on the morphological and physiological responses of plants when exposed to specific wavelengths and intensities of light. This study aimed to determine the effects of red, white, and blue LED light on the micropropagation of pascuita (Euphorbia leucocephala Lotsy), an ornamental plant native to Mexico and Central America.
Material and methods:
Under in vitro conditions, the effects of the above mentioned three LED light colors at 90 µmol m–2/s–1 intensity were evaluated on callus formation, shoot induction, and plant growth. In the second phase, using the shoots developed in the previous stage, the influence of the three LED light colors on in vitro root induction was evaluated with or without indole-3-acetic acid (IAA) addition.
Results:
Red light enhanced callus induction and growth, with plant responses observed from the initial days of culture, while blue light favored shoot formation. During the rooting phase, red light demonstrated superior efficacy by inducing the highest number of roots, achieving a 100% rooting rate, regardless of IAA presence.
Conclusions:
The use of different LED light colors during pascuita micropropagation is an effective tool to modulate plant responses throughout the various stages of regeneration.
The application of different light emitting diodes (LED) light colors in plant micropropagation is gaining prominence based on the morphological and physiological responses of plants when exposed to specific wavelengths and intensities of light. This study aimed to determine the effects of red, white, and blue LED light on the micropropagation of pascuita (Euphorbia leucocephala Lotsy), an ornamental plant native to Mexico and Central America.
Material and methods:
Under in vitro conditions, the effects of the above mentioned three LED light colors at 90 µmol m–2/s–1 intensity were evaluated on callus formation, shoot induction, and plant growth. In the second phase, using the shoots developed in the previous stage, the influence of the three LED light colors on in vitro root induction was evaluated with or without indole-3-acetic acid (IAA) addition.
Results:
Red light enhanced callus induction and growth, with plant responses observed from the initial days of culture, while blue light favored shoot formation. During the rooting phase, red light demonstrated superior efficacy by inducing the highest number of roots, achieving a 100% rooting rate, regardless of IAA presence.
Conclusions:
The use of different LED light colors during pascuita micropropagation is an effective tool to modulate plant responses throughout the various stages of regeneration.
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| ISSN: | 0860-7796 |
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