The suitability of in vitro culture technique for vegetative propagation of Biscutella laevigata calamine ecotype
was investigated. Various organic additives were tested as medium supplements: squash obtained from fleshy
pineapple fructification, liquid endosperm of coconut, and a conditioned medium acquired from the culture of
green algae. Exploited plant-derived biostimulators along with growth regulators included in the propagation
medium, improved B. Laevigata multiplication in vitro. The highest micropropagation coefficient, exceeding three
adventitious rosettes from one maternal microcutting, was obtained in the medium containing 10 ml A l^-1 pineapple
pulp during a 6-week-long culture. Microrosettes were rooted in vitro in a hormone-free, modified propagation
medium, with macro- and microelements accounting for one third of the initial concentration. Acclimatization
to ex vitro environment was conducted both in growth chamber and greenhouse conditions. Plants cultivated
in the greenhouse bloomed and set seed. The results of the experiment allowed determination of the most
suitable conditions required for culture initiation and successful development of a protocol for in vitro propagation
of Biscutella laevigata ecotype obtained from the Olkusz district.