RESEARCH PAPER
Embryogenic callus formation by cotyledon
and leaf explants of Crambe abyssinica seedlings
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Publication date: 2014-10-28
BioTechnologia 2011;92(2):209-213
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ABSTRACT
The study investigates the regeneration capacity of Crambe abyssinica via the callus phase. The conducted experiments
provide an overview of various conditions appropriate for callus development and its subsequent differentiation.
Explants from cotyledons and leaves of sterile 14-day-old seedlings of C. abyssinica cv. Mayer were used.
The primary callus so obtained was subsequently utilised for the observation of embryogenesis. The best callus
formation on the explants was achieved on the agar-solidified MS medium with 0.5 mg ·dm-3 NAA and 0.5 mg ·dm-3
BAP or TDZ. The formation of secondary embryogenic callus was most efficient on MS medium supplemented
with 0.4 mg ·dm-3 NAA and 0.3 mg ·dm-3 of BAP.