RESEARCH PAPER
In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants
1
Department of Horticulture Science, Ramin University of Agriculture and Natural Resources, Khuzestan, Iran
2
Department of Agronomy and Plant Breeding, Ramin University of Agriculture and Natural Resources, Khuzestan, Iran
Submission date: 2016-12-23
Final revision date: 2017-04-07
Acceptance date: 2017-05-11
Publication date: 2017-07-18
BioTechnologia 2017;98(2):113-119
KEYWORDS
TOPICS
ABSTRACT
Passiflora caerulea L. is a herbaceous climber that belongs to the Passifloraceae family. One of the most important techniques used in plant biotechnology is tissue culture, which allows for the mass production of pathogenfree plants. Cotyledonary nodes have a great potential for shoot proliferation; however, to the best of our knowledge there are no reports regarding plant regeneration from cotyledonary nodes of P. caerulea. Therefore, this study aimed to evaluate the potential of two different types of explants (shoot tips and cotyledonary nodes) to obtain shoot multiplication of P. caerulea. Various concentrations of 6-benzylaminopurine (BAP) (0.5, 1, and 1.5 mg/l), 6-furfurylaminopurine (kinetin, KIN) (1 and 2 mg/l), and thidiazuron (TDZ) (0.25, 0.5, and 1 mg/l) in combination with indole butyric acid (IBA) were used in a completely randomized design, in three replications. The results showed that the highest percentage of regeneration frequency (90%) and a maximum number of shoots (8.86) in cotyledonary node explants were obtained on MS medium supplemented with 1.5 mg/l BAP along with 0.15 mg/l IBA. Furthermore, in the shoot tip explants, the percentage of regeneration rate (96.66%) and the highest number of shoots (9.86) were obtained in the above-mentioned medium. In rooting experiments, the maximum rooting percentage (90%) was obtained on MS medium containing 1 mg/l IBA. In vitro-raised plantlets were placed in pots and were stored in soil under room temperature for 20 to 30 days before planting, and it showed more than 90% survival rate. Based on our results, the protocol described in this study has a high potential to be used in the micropropagation of this valuable plant.
REFERENCES (36)
1.
Anand S.P., Jayakumar E., Jeyachandran R., Nandagobalan V., Doss A. (2012) Direct organogenesis of Passiflora foetida L. through Nodal Explants. Plant Tiss. Cult. Biotechnol. 22(1): 87-91.
2.
Bellamine J., Penel C., Greppin H., Gaspar T. (1998) Confirmation of the role of auxin and calcium in the late phases of adventitious root formation. Plant Growth Regul. 26(3): 191-194.
3.
Busilacchi H., Severin C., Gattuso M., Aguirre A., Di Sapio O., Gattuso S. (2008) Field culture of micropropagated Passiflora caerulea L. histological and chemical studies. Bol. Latinoam. Caribe. 7: 257-263.
4.
Darshini R., Trivedi D.R., Joshi A.G. (2014) In vitro shoot regeneration of Stereospermum suaveolens using cotyledonary node and nodal explants. Plant Tissue Cult. Biotechnol. 24(2): 235-246.
5.
Da Silva C.V., De Oliveira L.S., Loriato V.A.P., Da Silva L.C., De Campos J.M.S., Viccini L.F., Otoni W.C. (2011) Organogenesis from root explants of commercial populations of Passiflora edulis Sims. and a wild passion fruit species, Passiflora cincinnata Masters. Plant Cell Tiss. Organ. Cult. 107(3): 407-416.
6.
Dobránszki J., Da Silva T.J.A. (2010) Micropropagation of an apple a review. Biotechnol. Adv. 28: 462-488.
7.
Gaspar T.H., Kevers C., Penel C., Greppin H., Reid D.M., Thorpe T.A. (1996) Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cell. Dev. Biol. Plant. 32(4): 272-289.
8.
Gattuso S., Severin C., Salinas A., Gattuso M., Giubileo G., Aguirre A., Busilacchi H. (2003) Micropropagation of Passiflora caerulea L. and histological studies of tissue regeneration. J. Trop. Medic. Plant. 4(2): 249-256.
9.
Gioria R., Bosquę G.G., Rezende J.A.M., Amorim L., Kitajima E.W. (2000) Incidencia de viroses de maracujazeiro na Alta Paulista – SP e danos causados pelo “Passion fruit woodiness virus”. Fitopatol. Bras. 25: 182-189.
10.
Gomez-Leyva J.F., Martinez-Acosta L.A., Lopez Muraira I.G., Silos-Espino H., Ramirez-Cervantes F., Andrade-Gonzalez I. (2008) Multiple shoot regeneration of roselle (Hibiscus sabdariffa L.) from a shoot apex culture system. Int. J. Bot. 4: 326-330.
11.
Hesami M., Daneshvar M.H. (2016) Development of a regeneration protocol through indirect organogenesis in Chenopodium quinoa Willd. Indo-Am. J. Agric. Vet. Sci. 4(1): 25-32.
12.
Jafari M., Daneshvar M.H., Lotfi A. (2016) Control of in vitro contamination of Passiflora caerulea by using of sodium Hypochlorite. Indo-Am. J. Agric. Vet. Sci. 4(2): 8-15.
13.
Kollmeier M., Mafelle H.H., Horst W.J. (2000) Is basipetal auxin flow Involved in inhibition of root elongation. Plant Physiol. 122: 945-956.
14.
Lee M., Phillips R.L. (1987) Genomic rearrangements in maize induced by tissue culture. Genome 29: 122-128.
15.
Ludwig-Muller J. (2000) Indole-3-butyric acid in plant growth and development. Plant Growth Regul. 32: 219-230.
16.
Montanher A.B., Zucolotto S.M., Schenkel E.P., Frode T.S. (2007) Evidence of anti-inflammatory effects of Passiflora edulis in an inflammation model. J. Ethnopharmacol. 109: 281-288.
17.
Mozaffarian V.A. (1996) Dictionary of Iranian Plant Names. Tehran: Farhang Moaser Press.
18.
Murashige T. (1980) Plant growth substances in commercial uses of tissue culture. [in:] Growth Substances. Ed. Skoog F., Berlin, Heidelberg: Springer-Verlag: 426-434.
19.
Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473- 497.
20.
Nascimento K.S., Ferreira K.T. (2012) Estabelecimento in vitro tissue culture maracujá silvestre (Passiflora foetida L.). Rev. Bras. Plant. Med. 14: 138-142.
21.
Nhut D.T., Khiet B.L.T., Thi N.N., Thuy D.T.T., Duy N., Hai N.T., Huyen P.X. (2007) High-frequency shoot formation of yellow passion fruit (Passiflora edulis f. flavicarpa) via thin cell layer (TCL) technology. [in:] Protocols for Micropropagation of Woody Trees and Fruits. Ed. Jain S.M., Häggman H., Berlin, Heidelberg: Springer-Verlag: 417-426.
22.
Ning G., Bai S., Bao M., Liu L. (2007) Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume “Xue mei”. In Vitro Cell. Dev. Biol. Plant. 43: 95-100.
23.
Ozarowski M., Thiem B., Gryszczynska A., Budzianowski J. (2013) Studies on in vitro seed germination and plant regeneration from the mature leaf, internodal and petiole explants of Passiflora caerulea L. Bot. Sci. 23(6): 937-947.
24.
Pasternak T., Miskolczi P., Ayaydin F., Mészáros T., Dudits D., Fehér A. (2000) Exogenous auxin and cytokinin-dependent activation of CDKs and cell division in leaf protoplastderived cells of alfalfa. Plant Growth Regul. 32: 129-141.
25.
Parveen S., Shahzad A. (2010) TDZ-induced high-frequency shoot regeneration in Cassia sophera L. via cotyledonary node explants. Physiol. Mol. Biol. Plants. 16(2): 201-206.
26.
Pati P.K., Rath S.P., Sharma M., Sood A., Ahuja P.S. (2006) In vitro propagation of rose – a review. Biotechnol. Adv. 24(1): 94-114.
27.
Purohit S.D., Dave A. (1996) Micropropagation of Sterculia urens Roxb. An endangered tree species. Plant Cell Rep. 15: 704-706.
28.
Pradhan C., Kar S., Pattnaik S., Chand P.K. (1998) Propagation of Dalbergia sissoo Roxb. through in vitro shoot proliferation from cotyledonary nodes. Plant Cell Rep. 18: 122-126.
29.
Prithviraj H.S., Hemanth K., Prakasha N.K., Shobha J. (2015) An efficient in vitro regeneration of multiple shoots from leaf explant of passiflora caerulea L. an important medicinal plant. Int. J. Recent. Sci. Res. 6(11): 7263-7265.
30.
Ragavendran C., Kamalanathan D., Reena G., Natarajan D. (2012) In vitro propagation of nodal and shoot tip explants of Passiflora foetida L. An exotic medicinal plant. Asian. J. Plant. Sci. Res. 2: 707-711.
31.
Sammaiah D., Shekar C., Goud M.J.P., Reddy K.J. (2011) In vitro selection of Pesticide tolerance and regeneration of plantlets in Solanum melongena L. J. Microbiol. Biotechnol. Res. 1(1): 66-70.
32.
Shekhawat M.S., Manokari M., Ravindran C.P. (2015) An improved micropropagation protocol by ex vitro rooting of Passiflora edulis sims. f. flavicarpa deg. through nodal segment culture. Scientifica Doi/10.1155/2015/578676.
33.
Siwach P., Gill A.R. (2011) Enhanced shoot multiplication in Ficus religiosa L. in the presence of adenine sulfate, glutamine, and phloroglucinol. Physiol. Mol. Biol. Plant. 17(3): 271-280.
34.
Techato S., Lim M. (2000) Improvement of mangosteen micropropagation through meristematic nodular callus formation from in vitro-derived leaf explants. Sci. Hort. 86: 291-298.
35.
Vieira L.M., Rocha D.I., Taquetti M.F., Da Silva L.C., De Campos J.M.S., Viccini L.F., Otoni, W.C. (2014) In vitro plant regeneration of Passiflora setacea (Passifloraceae): the influence of explant type, growth regulators, and incubation conditions. In Vitro Cell. Dev. Biol. Plant. 50(6): 738-745.
36.
Yang J.L, Seong E.S, Kim M.J, Ghimire B.K, Kang W.H., Yu .Y., Li C.H. (2010) Direct somatic embryogenesis from pericycle cells of broccoli (Brassica oleracea L. var. italica) root explants. Plant Cell Tiss. Organ. Cult. 100: 49-58.
Share
RELATED ARTICLE
| eISSN: | 2353-9461 |
| ISSN: | 0860-7796 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
