The presented study demonstrates the influence of lunasin, a bioactive peptide isolated from seeds of winter triticale, on the proliferation of neoplastic cells. In order to conduct investigations, 2S fraction low-molecular-weight albumin proteins were extracted. SDS-PAGE electrophoresis separation of extracts indicated the presence of a 5 kDa peptide in the crude extract, which corresponded to the weight of lunasin. The next stage of protein purification involved ion-exchange column chromatography in an FPLC system on DEAE Sepharose Fast Flow. The separation of biomolecules by means of 2D electrophoresis confirmed that a peptide with an isoelectric point was present in the area, with a pH of about 5.5 and molecular mass of about 5 kDa. The presence of lunasin in the purified samples was confirmed by Western blot. The lunasin peptide affected the proliferation of cervical cancer (HeLa) and ovarian cancer (SK-OV-3) cell lines. The average decrease in the HeLa cell proliferation rate was 17%. The SK-OV-3 cell line was more susceptible to inhibition by the protein fraction containing lunasin than HeLa cells, with an average decrease in the proliferation rate of 48%.
REFERENCES(21)
1.
Alaswad A.A., Krishnan H.B. (2016) Immunological investigation for the presence of lunasin, a chemopreventive soybean peptide, in the seeds of diverse plants. J. Agric. Food Chem 64: 2901-2909.
Cam A., de Mejia E.G. (2012) RGD-peptide lunasin inhibits Akt-mediated NF-κB activation in human macrophages through interaction with the αVβ3 integrin. Mol. Nutr. Food Res. 56: 1569-1581.
De Mejia E.G., Wang W., Dia V.P. (2010) Lunasin, with an arginine-glycine-aspartic acid motif, causes apoptosis to L1210 leukemia cells by activation of caspase-3. Mol. Nutr. Food Res. 54: 406-414.
Galbas M., Porzucek F., Woźniak A., Słomski R., Selwet M. (2013) Isolation of low-molecular albumins of 2S fraction from soybean (Glycine max (L.) Merrill). Acta Biochim. Pol. 60: 107-110.
Galvez A.F., Chen N., Macasieb J., de Lumen B.O. (2001) Chemopreventive property of a soybean peptide (lunasin) that binds to deacetylated histones and inhibits acetylation. Cancer Res. 61: 7473-7478.
Garcia-Nebot M.J., Recio I., Hernandez-Ledesma B. (2014) Antioxidant activity and protective effects of peptide lunasin against oxidative stress in intestinal Caco-2 cells. Food Chem. Toxicol. 65: 155-161.
Hernández-Ledesma B., Hsieh C.C., de Lumen B.O. (2009a) Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264.7 macrophages. Biochem. Biophys. Res. Commun. 390: 803-808.
Hsieh C., Hernández-Ledesma B., de Lumen B.O. (2011) Lunasin, a new breast cancer chemopreventive seed peptide. [in:] Breast cancer current and alternative therapeutic modalities. Ed. Esra Gunduz Mahmet Gunduz. InTech. 11: 215-242.
Jeong H.J., Jeong J.B., Kim D.S., de Lumen B.O. (2007) Inhibition of core histone acetylation by the cancer preventive peptide lunasin. J. Agric. Food Chem. 55: 632-637.
Jeong H.J., Leea J.R., Jeong J.B., Park J.H., Cheong Y., de Lumen B.O. (2009) The cancer preventive seed peptide lunasin from rye is bioavailable and bioactive. Nutr. Cancer. 61: 680-686.
Jeong J.B., De Lumen B.O., Jeong H.J. (2010a) Lunasin peptide purified from Solanum nigrum L. protects DNA from oxidative damage by suppressing the generation of hydroxyl radical via blocking fenton reaction. Cancer Lett. 293: 58-64.
Jeong H.J, Jeong J.B., Hsieh C.C., Hernández-Ledesma B., de Lumen B.O.(2010b) Lunasin is prevalent in barley and is bioavailable and bioactive in in vivo and in vitro studies. Nutr. Cancer. 62: 1113-1119.
Nakurte I., Klavins K., Kirhnere I., Namniece J., Adlere L., Matvejevs J., Kronberga A., Kokare A., Strazdina V., Legzdina L., Muceniece R. (2012) Discovery of lunasin peptide in triticale (X Triticosecale Wittmack). J. Cereal Sci. 56: 510-514.
Nakurte I., Kirhnere, I., Namniece J., Saleniece K., Krigere L., Mekss P., Vicupe Z., Bleidere M., Legzdina L., Muceniece R. (2013) Detection of the lunasin peptide in oats (Avena sativa L). J. Cereal Sci. 57: 319-324.
Seber L.E., Barnett B.W., McConnell E.J., Hume S.D., Cai J. (2012) Scalable purification and characterization of the anticancer lunasin peptide from soybean. PLoS ONE 7(4): e35409.
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