RESEARCH PAPER
In silico and In vitro cytotoxic effect of prodigiosin-conjugated
silver nanoparticles on liver cancer cells (HepG2)
1
Drug Radiation Research Department, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT),
Atomic Energy Authority, Cairo, Egypt
2
Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo, Egypt
3
Bioinformatics Center, Faculty of Science, Helwan University, Cairo, Egypt
4
Nanotechnology Center, Helwan University, Cairo, Egypt
Submission date: 2017-03-17
Final revision date: 2017-06-29
Acceptance date: 2017-07-25
Publication date: 2018-01-02
BioTechnologia 2017;98(3):225-243
KEYWORDS
TOPICS
Small organic compoundsOther - medical biotechnologyBiomanufacturingOther - industrial biotechnologyStructure prediction
ABSTRACT
This study demonstrates a novel approach for the synthesis of silver nanoparticles (AgNPs) against human liver cancer cell line (HepG2) using prodigiosin pigment isolated from Serratia marcescens. It further investigates the influence of various parameters such as initial pH, temperature, silver nitrate (AgNO3) concentration, and prodigiosin concentration on stability and optical properties of synthesized prodigiosin AgNPs. Highly stable, spherical prodigiosin-conjugated AgNPs were synthesized with a mean diameter of 9.98 nm using a rapid one-step method. The cytotoxic activity investigated in the present study indicated that prodigiosin and prodigiosin-conjugated AgNPs possessed a strong cytotoxic potency against human liver cancer. The In silico molecular docking results of prodigiosin and prodigiosin-conjugated AgNPs are congruent with the In vitro studies and these AgNPs can be considered as good inhibitors of mitogen-activated protein kinase 1 (MEK kinases). The study opened the possibility of using prodigiosin-conjugated AgNPs to increase the efficiency of liver cancer treatment.
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| ISSN: | 0860-7796 |
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