RESEARCH PAPER
Optimizing biomethanation of a lignocellulosic biomass
using indigenous microbial-cellulases systems
1
Department of Microbiology, Federal University of Technology, Owerri, Nigeria
2
Department of Chemistry, Federal University of Technology, Owerri, Nigeria
Submission date: 2017-03-03
Final revision date: 2017-06-29
Acceptance date: 2017-07-24
Publication date: 2018-01-02
BioTechnologia 2017;98(3):245-255
KEYWORDS
TOPICS
ABSTRACT
Studies on enhancing biomethanation were performed to ascertain whether amending lignocellulosic biomass waste with indigenous microbial-cellulases systems will improve biomethane output. To evaluate this, gastrointestinal contents of slaughtered beef cattle were treated with inocula derived from the guts of giant African land snail (Archachatina marginata ) and worker termites (Coptotermes formosanus ), individually as well as combined. The fed-batch method operating at prevailing ambient room temperatures (30 ± 2EC) for a hydraulic retention time (HRT) of 60 days was adopted. Feedstock slurry without amendment, amended with Archachatina marginata -derived inoculum, amended with Coptotermes formosanus -derived inoculum, and amended with Archachatina marginata: Coptotermes formosanus (50 : 50%) mixed inocula yielded cumulative biomethane of 65.26 ml/g VS, 63.21 ml/g VS, 125.99 ml/g VS, and 97.16 ml/g VS, respectively. Physicochemical analysis of feedstock and digestates revealed increased reductions in lignin, hemicelluloses, and celluloses (lignocelluloses) in trials amended with microbial-cellulases systems. This study revealed that among the experiments assayed, the trial amended with the cellulases system from Coptotermes formosanus yielded the highest cumulative biomethane.
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| eISSN: | 2353-9461 |
| ISSN: | 0860-7796 |
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