SHORT COMMUNICATION
Temporal expression of three conserved putative microRNAs
in response of Citrus × Limon to Xanthomonas citri subsp. citri
and Xanthomonas fuscans subsp. Aurantifolii
1
Shahid Beheshti University, Tehran, Iran
2
Oil Seeds Research Department, Seed and Plant Improvement Institute, Karaj, Iran
Submission date: 2016-12-26
Final revision date: 2017-07-03
Acceptance date: 2017-07-05
Publication date: 2018-01-02
BioTechnologia 2017;98(3):257-264
KEYWORDS
TOPICS
ABSTRACT
Citrus canker is a widespread bacterial disease that severely affects Citrus production. Xanthomonas spp. are the causative agents of the disease, which manifests as necrotic pustule-like lesions on the plant organs including leaves, fruits, and stems. MicroRNAs (miRNAs) are well-established post-transcriptional gene expression regulators that modulate plant responses during many stress conditions. Accordingly, some bacteria-responsive miRNAs have been shown to have regulatory functions in the plant-pathogen interactions. In this study, we successfully assessed the expression patterns of three conserved miRNAs: miR159, miR167, and miR398, in Citrus × Limon (lemon) infiltrated with Xanthomonas strains A and C using stem-loop RT-qPCR. Our results showed that the expressions of miR159 and mir167 have nearly similar patterns upon inoculations, while the expression of miR398 remains constantly up-regulated after an early induction.
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| eISSN: | 2353-9461 |
| ISSN: | 0860-7796 |
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