REVIEW PAPER
Isolation and characterization of biosurfactants-producing
bacteria isolated from palm oil industry and evaluation
for biosurfactants production using low-cost substrates
Publication date: 2014-10-23
BioTechnologia 2013;94(3):275-284
KEYWORDS
Isolationbiosurfactantrenewable substratephylogenetic analysispalm oil contaminated soilsurface tension
ABSTRACT
Biosurfactants-producing bacteria were isolated from various palm oil refinery industrial sites in the south of Thailand.
Isolates were screened for biosurfactant production by using low-cost, agro-industrial by-products or wastes
as a substrate. Based on drop collapsing test and emulsification activity, 25 isolates were selected. All the selected
isolates reduced the growth medium surface tension to 40 mN/m and produced emulsions with xylene. Twenty
isolates exhibited high emulsion-stabilizing capacity, maintaining more than 50% of the original emulsion volume
for 24 h. The phylogenetic position of these 25 isolates was evaluated by 16S rRNA gene sequence analysis.The
production of biosurfactants was determined for strains representative of 15 different bacterial genera, six of them
(Azorhizobium, Buttiauxella, Comamonas, Halopenitus, Haloplanus and Sinorhizobium ) have been for the first time
reported in this study as biosurfactant-producing strains. Additionally, Sinorhizobium meliloti AS91 and Marinobacter
hydrocarbonoclasticus AS51 produced extracellular biosurfactant which exhibited the lowest surface tension
(32 mN/m) and emulsification activity (69%) when cashew apple juice and used vegetable oil were used as the carbon
source, respectively. Overall, this is the first study of a phylogenetic analysis of biosurfactant-producing bacteria
from palm oil refinery industry site and their ability to produce biosurfactant on renewable substrates.
Isolates were screened for biosurfactant production by using low-cost, agro-industrial by-products or wastes
as a substrate. Based on drop collapsing test and emulsification activity, 25 isolates were selected. All the selected
isolates reduced the growth medium surface tension to 40 mN/m and produced emulsions with xylene. Twenty
isolates exhibited high emulsion-stabilizing capacity, maintaining more than 50% of the original emulsion volume
for 24 h. The phylogenetic position of these 25 isolates was evaluated by 16S rRNA gene sequence analysis.The
production of biosurfactants was determined for strains representative of 15 different bacterial genera, six of them
(Azorhizobium, Buttiauxella, Comamonas, Halopenitus, Haloplanus and Sinorhizobium ) have been for the first time
reported in this study as biosurfactant-producing strains. Additionally, Sinorhizobium meliloti AS91 and Marinobacter
hydrocarbonoclasticus AS51 produced extracellular biosurfactant which exhibited the lowest surface tension
(32 mN/m) and emulsification activity (69%) when cashew apple juice and used vegetable oil were used as the carbon
source, respectively. Overall, this is the first study of a phylogenetic analysis of biosurfactant-producing bacteria
from palm oil refinery industry site and their ability to produce biosurfactant on renewable substrates.
REFERENCES (50)
1.
Achak M., Hafidi A., Ouazzani N., Sayadi S., Mandi L. (2009) Low cost biosorbent ‘‘banana peel’’ for the removal of phenolic compounds from olive mill wastewater: kinetic and equilibrium studies. J. Hazard. Mat. 166: 117-125.
2.
Arredondo H.I.V., Colorado R.A., Oliveira S. (2009) Ethanol production from banana fruit and its lignocellulosic residues: energy and renewability analysis. Int. J. Thermody. 12: 155-162.
3.
Bahlawane C., McIntosh M., Krol E., Becker A. (2008) Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility. Mol. Plant. Microbe. Interact. 21: 1498-1509.
4.
Banat I.M., Franzetti A., Gandolfi I., Bestetti G., Martinotti M.G., Fracchia L., Smyth T.J., Marchant R. (2010) Microbial biosurfactants production, applications and future potential. Appl. Microbiol. Biotechnol. 87: 427-444.
5.
Batista S.B., Mounteer A.H., Amorim F.R., Totola M.R. (2006) Isolation and characterization of biosurfactant/bioemulsifierproducing bacteria from petroleum contaminated sites. Bioresource Technol. 97: 868-875.
6.
Bento F.M., Camargo F.A., Okeke B.C., Frankenberger W.T. (2005) Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiol. Res. 160: 249-255.
7.
Bicca F.C., Fleck L.C., Ayub M.A.Z. (1999) Production of biosurfactant by hydrocarbon degrading Rhodococcus ruber and Rhodococcus erythropolis. Rev. Microbiol. 30: 231-236.
Bizet C., Barreau C., Harmant C., Nowakowski M., Pietfroid A. (1997) Identification of Rhodococcus, Gordona and Dietzia species using carbon source utilization tests (‘‘Biotype-100” strips). Res. Microbiol. 148: 799-809.
8.
Bodour A.A., Drees K.P., Raina M.M. (2003) Distribution of biosurfactant-producing bacteria in undisturbed and contaminated arid southwestern soils. Appl. Environ. Microbiol. 69: 3280-3287.
9.
Bossier P., Verstraete W. (1996) Comamonas testosteroni colony phenotype influences exopolysaccharide production and coaggregation with yeast cells. Appl. Environ. Microbiol. 62: 2687-2691.
10.
Bouffartigues E., Gicquel G., Bazire A., Fito-Boncompte L., Taupin L., Maillot O., Groboillot, A., Poc-Duclairoir C., Orange N., Feuilloley M., Dufour A., Chevalier, S. (2011) The major outer membrane protein OprF is required for rhamnolipid production in Pseudomonas aeruginosa. J. Bacteriol. Parasitol. 2: 118.
11.
Brito E.M.S., Guyoneaud R.M., Gońi-Urriza M., RanchouPeyruse A., Verbaere A., Crapez M.A.C., Wasserman J.C.S.A., Duran R. (2006) Characterization of hydrocarbonoclastic bacterial communities from mangrove sediments in Guanabara bay. Braz. Res. Microbiol. 157: 752-762.
12.
Burgos-Díaz C., Pons R., Espuny M.J., Aranda F.J., Teruel J.A., Manresa A., Ortiz A., Marqués A.M. (2011) Isolation and partial characterization of a biosurfactant mixture produced by Sphingobacterium sp. isolated from soil. J. Colloid. Interface. Sci. 361: 195-204.
Chavalparit O., Rulkens W.H., Mol A.P.J., Khaodhair S. (2006) Options for environmental sustainability of the crude palm oil industry in Thailand through enhancement of industrial ecosystems. Environ. Dev. Sustain. 8: 271-287.
13.
Chonga M.L., Rahima R.A., Shiraib Y., Hassana M.A. (2009) Biohydrogen production by
Clostridium butyricum EB6 from palm oil mill effluent. Int. J. Hydrogen. Energ. 34: 764-771. D’Haeze W., Glushka J., De Rycke R., Holsters M., Carlson R.W. (2004) Structural characterization of extracellular polysaccharides of Azorhizobium caulinodans and importance for nodule initiation on Sesbania rostrata. Mol. Microbiol. 52: 485-500.
14.
Das P., Mukherjee S., Sivapathasekaran S., Sen R. (2010) Microbial surfactants of marine origin: potentials and prospects. Adv. Exp. Med. Biol. 672: 88-101. Emaga T.H., Andrianaivo R.H., Wathelet B., Tchango J.T., Paquot M. (2007) Effects of the stage of maturation and varieties on the chemical composition of banana and plantain peels. Food Chem. 103: 590-600.
15.
Emaga T.H., Ronkart S.N., Robert C., Wathelet B., Paquot M. (2008) Characterisation of pectins extracted from banana peels (Musa Aaa) under different conditions using an experimental design. Food Chem. 108: 463-471.
16.
Essien J.P., Akpan E.J., Essien E.P. (2005) Studies on mould growth and biomass production using waste banana peel. Bioresource Technol. 96: 1451-1456.
17.
Franzetti A., Gandolfi I., Bertolini V., Raimondi C., Piscitello M. (2011) Phylogenetic characterization of bioemulsifierproducing bacteria. Int. Biodeter. Biodegr. 65: 1095-1099.
Guerin T.F. (2002) Heavy equipment maintenance wastes and environmental management in the mining industry. J. Environ. Manage. 66: 185-199.
18.
Haba E., Espuny M.J., Busquets M., Manresa A. (2000) Screening and production of rhamnolipids by Pseudomonas aeruginosa 47T2 NCIB 40044 from waste frying oils. J. Appl. Microbiol. 88: 379387. Holt J.G., Kreig N.R., Sneath P.H.A., Stanley J.T., William S.T. (1994) Bergey’s manual determinative bacteriology. William and Wilkins, Baltimore. Honorato T.L., Rabelo M.C., Goncalves L.R.B., Pinto C.A.S., Rodrigues S. (2007) Fermentation of cashew apple juice to produce high added value products. World. J. Microbiol. Biotechnol. 23: 1409-1415.
19.
Hossain M.A., Ngo H.H., Guo W.S., Nguyen T.V. (2012) Biosorption of Cu(II) from water by banana peel based biosorbent: experiments and models of adsorption and desorption. J. Water Sustain. 2: 87-104.
20.
Jachimska B., Lunkenheimer K., Malysa K. (1995) Effect of position of the functional group on the equilibrium and surface properties of butyl alcohols. J. Colloid. Interf. Sci. 176: 31-38.
21.
Karthikeyan A., Sivakumar N. (2010) Citric acid production by koji fermentation using banana peel as a novel substrate. Bioresource Technol. 101: 5552-5556.
22.
Klein B., Bouriat P., Goulas P., Grimaud R. (2010) Behavior of Marinobacter hydrocarbonoclasticus SP17 cells during initiation of biofilm formation at the alkane-water interface. Biotechnol. Bioeng. 105: 461-468.
23.
Kokab S., Asghar M., Rehman K., Asad M.J., Adedyo O. (2003) Bio-processing of banana peel for α-amylase production by Bacillus subtilis. Int. J. Agricul. Biol. 5: 36-39.
24.
Li J.L., Chen B.H. (2009) Surfactant-mediated biodegradation of polycyclic aromatic hydrocarbons. Mater. 2: 76-94.
25.
Ma A.N. (2000) Environmental management for the palm oil industry. Palm. Oil. Dev. 30: 1-9.
26.
Makkar R.S., Cameotra S.S. (2002) An update on the use of unconventional substrates for biosurfactant production and their new application. Appl. Microbiol. Biotechnol. 58: 428-434.
27.
Memon J.R., Memon S.Q., Bhanger M.I., El-Turki A., Hallam K.R., Allen G.C. (2009) Banana peel: a green and economical sorbent for the selective removal of Cr(VI) from industrial wastewater. Colloid. Surf. B. 70: 232-237.
28.
Mulligan C.N. (2009) Recent advances in the environmental applications of biosurfactants. Curr. Opin. Colloid In. 14: 372-378. Nawawi W.M.F.W., Jamal P., Alam M.Z. (2010) Utilization of sludge palm oil as a novel substrate for biosurfactant production. Bioresource Technol. 101: 9241-9247.
29.
Neelakandan T., Usharani G. (2009) Optimization and production of bioethanol from cashew apple juice using immobilized yeast cells by Saccaharomyces cerevisiae. Am-Eur. J. Scic. Res. 4: 85-88. Nerurkar A.S., Hingurao K.S., Suthar H.G. (2009) Bioemulsifiers from marine microorganisms. J. Sci. Ind. Res. 68: 273-277.
30.
Ng W.K., Wang Y. (2011) Inclusion of crude palm oil in the brood stock diets of female Nile tilapia, Oreochromis niloticus, resulted in enhanced reproductive performance compared to brood fish fed diets with added fish oil or linseed oil. Aquaculture. 314: 122-131.
31.
Nitschke M., Coast S.G. (2007) Biosurfactants in food industry. Trends Food Sci. Technol. 18: 252-259.
32.
Osma J.F., Herrera J.L.T., Couto S.R. (2007) Banana skin: a novel waste for laccase production by trametes pubescens under solid-state conditions. application to synthetic dye decolouration. Dyes Pigments. 75: 32-37.
33.
Patil S.N., Aglave B.A., Pethkar A.V., Gaikwad V.B. (2012) Stenotrophomonas koreensis a novel biosurfactant producer for abatement of heavy metals from the environment. Afr. J. Microbiol. Res. 6: 5173-5178.
34.
Perfumo A., Smyth T.J.P., Marchant R., Banat I.M. (2010) Production and roles of biosurfactants and bioemulsifiers in accessing hydrophobic substrates. In: Timmis KN, editor. Handbook of hydrocarbon and lipid microbiology. Springer-Verlag, Berlin, Germany. p. 1501-1512.
35.
Plaza G.A., Zjawiony I., Banat I.M. (2006) Use of different methods for detection of thermophilic biosurfactant-producing bacteria from hydrocarbon contaminated and bioremediated soils. J. Pet. Sci. Eng. 50: 71-77.
36.
Poli A.,Donato P.D., Abbamondi G.R.,Nicolaus B. (2011) Synthesis, production, and biotechnological applications of exopolysaccharides and polyhydroxyalkanoates by archaea. Archaea. doi:10.1155: 693-253.
37.
Ruggeri C., Franzetti A., Bestetti G., Caredda P., La Colla P., Pintus M., Sergi S., Tamburini E. (2009) Isolation and characterization of surface active compound-producing bacteria from hydrocarbon-contaminated environments. Int. Biodeter. Biodegr. 63: 936-942.
38.
Saimmai A., Rukadee O., Onlamool T., Sobhon V., Maneerat S. (2012a) Characterization and phylogenetic analysis of microbial surface active compounds-producing bacteria. Appl. Biochem. Biotech. 168: 1003-1018.
39.
Saimmai A., Sobhon V., Maneerat S. (2012b) Production of biosurfactant from a new and promising strain of Leucobacter komagatae 183. Ann. Microbiol. 62: 391-402.
40.
Saimmai A., Tani A., Sobhon V., Maneerat S. (2012c) Mangrove sediment, a new source of potential biosurfactant producing bacteria. Ann. Microbiol. 62: 1669-1679.
41.
Satpute S.K., Banat I.M., Dhakephalkar P.K., Banpurkar A.G., Chopade B.A. (2010) Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnol. Adv. 28: 436-450.
42.
Tamura K., Dudley J., Nei M., Kumar S. (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596-1599.
43.
Thompson J.D., Gibbons T.J., Plewniak F., Jeanmougin F., Higgins D.G. (1997) The CLUSTAL X windows interfac: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882.
44.
Toledo F.L., Calvo C., Rodelas B., Gonzalez-Lopez J. (2006) Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities. Syst. Appl. Microbiol. 29: 244-252.
45.
Toledo F.L., Gonzalez-Lopez J., Calvo C. (2008) Production of bioemulsifier by Bacillus subtilis, Alcaligenes faecalis and Enterobacter species in liquid culture, Bioresource Technol. 99: 8470-8475.
46.
Turner S., Pryer K.M., Miao V.P.W., Palmer J.D. (1999) Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. J. Eukaryot. Microbiol. 46: 327-338.
47.
Wong Y.M., Brigham C.J., Rha C., Sinskey A.J., Sudesh K. (2012) Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator. Bioresource Technol. 121: 320-327.
48.
Yakimov M.M., Timmis K.N., Golyshin P.N. (2007) Obligate oil-degrading marine bacteria. Curr. Opin. Biotech. 18: 257-266.
49.
Yang F., Hanna M.A., Sun R. (2012) Value-added uses for crude glycerol a by-product of biodiesel production. Biotechnol. Biofuel. 5:13.
50.
Youssef N.H., Dunacn K.E., Nagle D.P., Savage K.N., Knapp R.M., McInerney M.J. (2004) Comparison of methods to detect biosurfactant production by diverse microorganism. J. Microbiol. Meth. 56: 339-347.
| eISSN: | 2353-9461 |
| ISSN: | 0860-7796 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
