RESEARCH PAPER
Obtaining doubled haploid lines of the Lr19 gene using anther cultures of winter wheat genotypes
1
Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Poland
2
Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Poznań, Poland
3
DANKO Plant Breeding Ltd.
Submission date: 2016-04-29
Final revision date: 2016-10-12
Acceptance date: 2016-10-13
Publication date: 2017-01-13
BioTechnologia 2016;97(4):285-293
KEYWORDS
TOPICS
ABSTRACT
Leaf rust pathogens are able to overcome plants’ resistance by the appearance of new races. Therefore, an introduction of leaf rust resistance genes into highyielding wheat genotypes is essential. One of such genes is Lr19 (leaf rust resistance gene) that could be a source of resistance in polish breeding programs. The aim of this study was to obtain doubled haploid lines of the Lr19 gene in anther cultures of winter wheat genotypes. In the experiment, F3 generation plants from two cross-combinations were used: genotype T39 × Ozon and genotype T36 × Hondia (T36 and T39 lines carry Lr19 gene). Three combinations of growth regulators such as 2,4-D; 2,4-D and dicamba; and 2,4-D and kinetin were applied. A total of 13,550 anthers were tested. The embryo-like structures were formed at an average frequency of 9.95% and were mainly influenced by the genotype, not by the composition of hormones in induction media. Regeneration frequency of green plants was recorded to be 1.45%. The
highest frequency of green plant regeneration was observed on induction media supplemented with 2,4-D and dicamba reaching 1.79%. Spontaneous doubled haploids tested by laser flow cytometry were detected in 33.95% of plants. The frequency of the formation of embryo-like structure was mainly influenced by the genotype, not by the composition of hormones in induction media.
highest frequency of green plant regeneration was observed on induction media supplemented with 2,4-D and dicamba reaching 1.79%. Spontaneous doubled haploids tested by laser flow cytometry were detected in 33.95% of plants. The frequency of the formation of embryo-like structure was mainly influenced by the genotype, not by the composition of hormones in induction media.
REFERENCES (36)
1.
Baenziger S., Kim K.M., Haliloglu K. (2001) Wheat in vitro breeding. The world wheat book: A history of wheat breeding. Intercept Limagrain New York: 979-1000.
2.
Barnabas B., Pfahler P.L., Kovacs G.(1991) Direct effect of colchicine on microspore embryogenesis to produce dihaploid plant in wheat (Triticum aestivum L.). Theor. Appl. Genet. 81: 675-678.
3.
Barnabas B., Szakacs E., Karsai I., Bedö Z. (2001) In vitro androgenesis of wheat: from fundamentals to practical application. Euphytica 119: 211-216.
4.
Broughton S. (2011) The application of n-butanol improves embryo and green plant production in anther culture of Australian wheat (Triticum aestivum L.) genotypes. Crop Pasture Sci. 62: 813-822.
5.
Chaudhary H.K., Dhaliwal I., Singh S., Sethi G.S. (2003) Genetics of androgenesis in winter and spring wheat genotypes. Euphytica 132: 311-319.
6.
Dobrovolskaya O.B., Pershina L.A., Kravstova L.A., Silkova O.G., Shchapova A.I. (2001) The effect of rye chromosomes on the characteristic features of androgenesis in wheat-rye substitution lines Triticum aestivum L. (cv. Saratovskaya 29)/ Secale cereal L. (cv. Onokhoiskaya) and Triticale. Russ. J. Genet. 37(5): 506-512.
7.
Dobrovolskaya O.B., Pershina L.A., Kravstova L.A., Shchapova A.I. (2003) Comparative effects of rye chromosomes 1R and 5R on androgenesis in cultured anthers of wheat-rye substitution lines as dependent on the line origin. Russ. J. Genet. 39(4): 467-470.
8.
Hussain M., Niaz M., Iqbal M., Iftikhar T., Ahmad J. (2012) Emasculation techniques and detached tiller culture in wheat x maize crosses. J. Agricult. Res. 50: 1-19.
9.
Kasha K.J. (2005) Chromosome doubling and recovery of doubled haploid plants. In Haploids in crop improvement II: 123-152. Springer Berlin Heidelberg.
10.
Kim K.M., Baenziger S.P., Rybczynski J.J. (2003) Characterization of ploidy levels of wheat microspore-derived plants using laser flow cytometry. In Vitro Cell. Dev. Biol.- Plant 39: 663-668.
11.
Kondić-Špika A.D., Kobiljski B.D., Hristov N.S. (2008) Efficiency of anther culture technique in the production of wheat double haploids. Proc. Nat. Sci. Matica Srpska Novi Sad. 115: 35-40.
12.
Kondić-Špika A., Vukosavljev M., Kobiljski B., Hristov N. (2011) Relationships among androgenetic components in wheat and their responses to the environment. J. Biol. Res. Thessal. 16: 217-223.
13.
Konieczny R., Bohdanowicz J., Czaplicki A.Z., Przywara L. (2005) Extracellular matrix surface network during plant regeneration in wheat anther culture. Plant Cell Tiss. Org. Cult. 83: 201-208.
14.
Kryczyński S., Weber Z. (red.). (2011) Fitopatologia. T. 2. Choroby roślin uprawnych. PWRiL, Poznań, 464 ss.
15.
Lantos C., Weyen J., Orsini, J.M., Gnad H., Schlieter B., Lein V., Kontowski S., Jacobi A., MihÁly R., Broughton S., Pauk J. (2013) Efficient application of in vitro anther culture for different European winter wheat (Triticum aestivum L.) breeding programmes. Plant Breed. 132: 149-154.
16.
Marciniak K., Kaczmarek Z., Adamski T., Surma M. (2003) The anther culture response of Triticale line x tester progenies. Cellular, Mol. Biol. Lett. 8: 343-351.
17.
Masojc P., Lukow O.M., McKenzie R.I.H., Howes N.K. (1993) Responsiveness to anther culture in cultivars and F1 crosses of spring wheat. Can. J. Plant Sci. 73: 777-783.
18.
McIntosh R.A., Wellings C.R., Park R.F. (1995) Wheat Rusts. An Atlas Of Resistance Genes. CSIRO, Australia: 1-86.
19.
McIntosh R.A., Yamazaki Y., Dubcovsky J., Rogers W.J., Morris C., Appels, R., Devos, K.M. (2010) Catalogue of gene symbols for wheat: 2010. In KOMUGI Integrated Wheat Science Database. Available online: www.shigen.nig.ac.jp/ wheat/komugi/genes (accessed 17 October 2012).
20.
Mendoza M.G., Kaeppler H.F. (2002) Auxin and sugar effects on callus induction and plant regeneration frequencies from mature embryos of wheat (Triticum aestivum L.). In Vitro Cell. Dev. Biol. – Plant 38: 39-45.
21.
Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15 (3): 473-497.
22.
Niroula R.K., Bimb H.P. (2009) Overview of 1 wheat x maize system of crosses for dihaploid induction in wheat. World Appl. Sci. J. 7: 1037-1045.
23.
Pauk, J., Kertész, Z., Beke, B., Bóna, L., Csősz, M., Matuz, J. (1995) New Winter Wheat Variety: 'GK Délibáb' Developed via Combining Conventional Breeding and In Vitro Androgenesis. Cereal Res. Commun. 23(3): 251-256.
24.
Pershina L.A., Osadchaya T.S., Badaeva E.D., Belan I.A., Rosseeva L.P. (2013) Androgenesis in anther cultures of cultivars and a promising form of spring common wheat of west Siberia differing in the presence or absence of wheat-alien translocations. Russ. J. Genet. Appl. Res. 17(1): 40-49.
25.
R Core Team. (2015). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Vienna, Austria. https://www.R-project.org.
26.
Redha A., Suleman P. (2011) Effects of exogenous application of polyamines on wheat anther cultures. Plant Cell Tiss. Organ Cult. 105: 345-353.
27.
Rybczyński J.J., Simonson R.L., Baezinger P.S. (1991) Evidence for microspore embryogenesis in wheat anther culture. In vitro Cell. Dev. Biol. 27: 168-174.
28.
Sibikeeva Y.E., Sibikeev S.N. (1996) Genetic analysis of anther culture response in wheat carrying alien translocations. Theoret. Appl. Genet. 92(6): 782-785.
29.
Sibikeeva Y.E., Sibikeev S.N., Krupnov V.A. (2004) The effect of Lr19-translocation on in vitro androgenesis and inheritance of leaf-rust resistance in DH3 lines and F2 hybrids of common wheat. Russ. J. Genet. 40(9): 1003-1006.
30.
Sibikeeva Y.E., Sibikeev S.N. (2014) The influence of combinations of alien translocations on in vitro androgenesis in near-isogenic lines of spring bread wheat. Russ. J. Genet. 50(7): 728-735.
31.
Śliwińska E. (2008) Estimation of DNA Content in Plants Using Flow Cytometry. Post. Biol. Komórki 35(24): 165- 176.
32.
Tadesse W., Inagaki M., Tawkaz S., Baum M., Van Ginkel M. (2012) Recent advances and application of doubled haploids in wheat breeding. Afr. J. Biotech. 11(89): 15484-15492.
33.
Torp A.M., Hansen A.I., Andersen S.B. (2001) Chromosomal regions associated with green plant regeneration in wheat (Triticum aestvum L.) anther culture. Euphytica 119: 377-387.
34.
Wang P, Chen Y. (1983) Preliminary study on production of height of pollen H2 generation in winter wheat grown in the field. Acta Agron. 9: 283-284.
35.
Weigt D., Nawracała J., Popowska D., Nijak K. (2012) Examination of ability to androgenesis of spring wheat genotypes resistant to Fusarium. BioTechnologia 93(2): 116-122.
36.
Zamani I., Kovacs G., Gouli-Vavdinoudi E., Roupakias D.G., Barnabas B. (2000) Regeneration of fertiledoubled haploid plants from colchicine supplemented media in wheat anther culture. Plant Breeding 119: 461-465.
| eISSN: | 2353-9461 |
| ISSN: | 0860-7796 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
