RESEARCH PAPER
Anther culture response and genetic relationships between Iranian and European barley (Hordeum vulgare L.) cultivars
 
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Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran
 
 
Submission date: 2017-01-12
 
 
Final revision date: 2017-08-07
 
 
Acceptance date: 2017-09-25
 
 
Publication date: 2018-03-16
 
 
BioTechnologia 2017;98(4):295-304
 
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ABSTRACT
Barley is a widely adaptable cereal grain, but with a low response from anther cultures to callus induction. In this study, the response to anther culture and the genetic diversity of 16 Iranian and 26 European barley cultivars were evaluated using 20 ISSR (Inter Simple Sequence Repeats) primers. The callus induction phase was investigated based on a completely randomized design with an unequal number of replications. The regeneration phase was studied using a factorial experiment based on a completely randomized design with 3 replications. The factors included the tested cultivars and several growth regulators. All cultivars that responded to anther cultures (8 of 42 cultivars) had European origin. A correlation analysis showed the highest correlation between the callus size and its growth rate. A cluster analysis based on anther culture traits grouped 8 cultivars into 2 classes. The effect of growth regulators was not significant for total regeneration ratio, green and albino plants ratio. The results showed that the efficiency of the anther culture method is largely dependent on the plant genotype. The percentage of polymorphism using ISSR markers ranged from 50 to 78.94. The range of genetic similarity coefficients among barley cultivars was from 0.138 to 0.867. According to the Mantel test, there was no significant correlation between the assumed variation patterns using ISSR molecular markers and anther culture data. The cluster analysis based on the ISSR markers divided 42 barley cultivars into 6 classes, although there was no clear pattern of clustering for differentiation between Iranian and European cultivars. Iranian cultivars showed a higher molecular variation than European ones.
REFERENCES (26)
1.
Abou-Deif M.H., Rashed M.A., Sallam M.A.A., Mostafa E.A.H., Ramadan W.A. (2013) Characterization of twenty wheat varieties by ISSR markers. Middle-East J. Sci. Res. 15: 168- 175.
 
2.
Chaghamirza K., Arzani A. (1999) Efficiency of potato media on androgenic response of Iranian wheat cultivars. Iran Agric. Res. 18: 21-30.
 
3.
Dellaporta S.L., Wood J., Hicks J.B. (1983) A plant DNA mini preparation: version II. Plant Mol. Biol. Rep. 1: 19-21.
 
4.
Eshghi R., Abrahimpour F., Ojaghi J., Salayeva S., Baraty M., Rahimi M. (2012) Evaluation of genetic variability in naked barley (Hordeum vulgare L.). Intl. J. Agri. Crop Sci. 4: 1166-1179.
 
5.
Fazeli F., Cheghamirza K. (2011) Evaluation of genetic diversity in Iranian chickpea (Cicer arietinum L.) accessions using ISSR markers. Mod. Genet. J. 6: 97-104.
 
6.
Fernandez M.E., Figueiras A.M., Benito C. (2002) The use of ISSR and RAPD marker for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor. Appl. Genet. 104: 845-851.
 
7.
Feug Z.Y., Liu X.J., Zhang Y.Z., Ling H.Q. (2006) Genetic diversity analysis of Tibetan wild barley using SSR markers. Acta Genet. Sin. 33: 917-928.
 
8.
Ghasemi Ghehsareh M., Salehi M., Khosh-Khui M., Niazi A. (2015) Application of ISSR markers to analyze molecular relationships in Iranian jasmine (Jasminum spp.) accessions. Mol. Biotechnol. 57: 65-74.
 
9.
Giancarla V., Emilian M., Radu S., Sorin C., Sorina P., Cerasela P. (2012) The use of RAPD and ISSR markers for genetic diversity among some barley cultivars. Rom. Biotech. Lett. 17: 7493-7503.
 
10.
Hunter C.P. (1988) Plant regeneration from microspores of barley (Hordeum vulgare L.). Dissertation: Wye College, University of London.
 
11.
Islam S., Tuteja N. (2012) Enhancement of androgenesis by abiotic stress and other pretreatments in major crop species. Plant Sci. 182: 134-144.
 
12.
Kahrizi D., Mahmoodi S., Bakhshi-Khaniki G.H., Mirzaei M. (2011) Effect of genotype on androgenesis in barley (Hordeom vulgare L.). Biharean biol. 5: 132-134.
 
13.
Khaidizar M.I., Haliloglu K., Elkoca E., Aydin M., Kantar F. (2012) Genetic diversity of common bean (Phaseolus vulgaris L.) landraces grown in northeast Anatolia of Turkey assessed with simple sequence repeat markers. Turk. J. Field Crops 17: 145-150.
 
14.
Komatsuda T., Pourkheirandish M., He C., Azhaguvel P., Kanamori H., Perovic D., Stein N., Graner A., Wicker T., Tagiri A., Lundqvist U., Fujimura T., Matsuoka M., Matsumoto T., Yano M. (2007) Six-rowed barley originated from a mutation in a homeodomain-leucine zipper I-class homeobox gene. Proc. Natl Acad. Sci USA 104: 1424-1429.
 
15.
Kumar M., Mishra G.P., Singh R., Kumar J., Naik P.K, Singh S.B. (2009) Correspondence of ISSR and RAPD markers for comparative analysis of genetic diversity among different apricot genotypes from cold arid deserts of trans- Himalaya. Physiol. Mol. Biol. Plants 15: 225-236.
 
16.
Makowska K., Oleszczuk S. (2014) Albinism in barley androgenesis. Plant Cell Rep. 33: 385-392.
 
17.
Maniruzzaman Z., Talukder Z.A., Rohman S., Begum F., Amiruzzaman M. (2014) Polymorphism study in barley (Hordeum vulgare L.) genotypes using microsatellite (SSR) markers. Bangladesh J. Agric. Res. 39: 33-45.
 
18.
Murashige T., Skoog F. (1962) A revised medium for rapid growth and bio agsays with tohaoco tissue cultures. Physiol. Plant. 15: 473-497.
 
19.
Najaphy A., Ashrafi Parchin R., Farshadfar E. (2011) Evaluation of genetic diversity in wheat cultivars and breeding lines using inter simple sequence repeat markers. Biotechnol. Biotechnol. Equip. 25: 2634-2638.
 
20.
Olgun M., Budakbasciftcil Z., Sciftcil U., Ayter N., Turan M., Koyuncu O., Ardic M., Agar G., Takil E. (2015) Genetic divergence in some barley (Hordeum vulgare L.) genotypes by RAPD and ISSR analyses. SDU Ziraat Fakultesi Dergisi 10: 102-109.
 
21.
Pradeep R.M., Sarla N., Siddiq E.A. (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128: 9-17.
 
22.
Prevost A., Wilkinson M.J. (1999) A new system of comparing PCR primers applied to ISSR ngerprinting of potato cultivars. Theor. Appl. Genet. 98: 107-112.
 
23.
Powell W., Morgante M., Andre C., Hanafey M., Vogel J., Tingey S., Rafalski A. (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breeding 2: 225-238.
 
24.
Shuorvazdi A., Mohammadi S. A., Norozi M., Sadeghzadeh B. (2014) Molecular analysis of genetic diversity and relationships of barley landraces based on microsatellite markers. J. Plant Genet. Res. 1: 51-64.
 
25.
Turasheva S.K. (2015) Production of wheat doubled haploids by in vitro anther culture. Int. J. Biol. Chem. 8: 21-25.
 
26.
Wright S. (1978) Evolution and the genetics of populations variability within and among natural populations. University of Chicago Press.
 
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ISSN:0860-7796
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