RESEARCH PAPER
Rapid callogenesis and plant regeneration of fine and coarse varieties of rice
 
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1
Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan
 
2
P.O. Box 7, Miki-cho post office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan
 
 
Submission date: 2017-03-23
 
 
Final revision date: 2017-07-22
 
 
Acceptance date: 2017-09-05
 
 
Publication date: 2018-03-16
 
 
BioTechnologia 2017;98(4):283-293
 
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ABSTRACT
In this study, we aimed to develop an effective and genotype-dependent protocol for in vitro callogenesis and plant regeneration of two fine rice varieties (Super Basmati and Basmati-515) and one coarse variety (KS-282). According to the results, maximum callus induction (52% of the explants) was observed in case of KS-282 after 11 days of incubation with Murashige and Skoog basal medium (MS, with 30 g/l sucrose and 1.76 g/l Gellan gum), which was supplemented with 3 mg/l of 2,4-dichlorophenoxy acetic acid (2,4-D). In contrast, 41% of the Super Basmati explants produced callus within 17 days, followed by 32% of the Basmati-515 explants (within 11 days), in the aforementioned medium but which is supplemented with 4 mg/l 2,4-D. Indirect shoot formation from callus was found to be the highest in case of KS-282 (24% of callus explants) and Basmati-515 (18% of callus explants) after 21 days of incubation. Next, we tested MS medium without 2,4-D but which was supplemented with 3 mg/l kinetin (Kin), 1 mg/l 6-benzyladenine (BA), and 0.5 mg/l indole-3-acetic acid (IAA). According to the results, 22% of the explants of Super Basmati formed shoots after 19 days on MS medium supplemented with BA instead of 2,4-D at a concentration of 1 mg/l. Roots were formed from shoots on a half-strength MS medium without plant growth regulators after 15 days in KS-282 (93% roots formed), after 19 days in Super Basmati (90% roots formed), and after 23 days in Basmati-515 (86% roots formed). Shoots were effectively multiplied in KS-282, followed by Super Basmati, and Basmati-515 varieties (37, 29, and 23 shoots/shoot, respectively) on a basal MS medium supplemented with 4 mg/l Kin. Rooting was induced within 47, 55, and 57 days for KS-282, Super Basmati, and Basmati-515, respectively. Following acclimatization, the percentage of survival was found to be 71%, 70%, and 65%, which was found to be at-par for all three tested rice varieties.
REFERENCES (36)
1.
Afrasiab H., Jafar. R. (2011) Effect of different media and solidifying agents on callogenesis and plant regeneration from different explants of rice (Oryza sativa L) varieties Super Basmati and IRRI-6. Pak. J. Bot. 43(1): 487-501.
 
2.
Akram M., Aftab F. (2012) Efficient micropropagation and rooting of king white mulberry (Morusmacroura Miq.) var. Laevigata from nodal explants of mature tree. Pak. J. Bot. 44: 285-289.
 
3.
Anonymous (2015-2016) Economic survey. Economic Survey of Pakistan. Finance and Economic Affairs Division, Ministry of Finance, Govt. Pakistan, Islamabad, Pakistan.
 
4.
Bano S., M. Jabeen F. Rahim., Ilahi I. (2005) Callus induction and regeneration in seed explants of rice (Oryza sativa cv. SWAT-II). Pak. J. Bot. 37(3): 829-836.
 
5.
Bhuiyan R., Abunasar M., Shakil S.K., Ikbal M.F., Mosnaz A.T.M.J., Hoque M.H., Biswas G.C., Prodhan S.H. (2014) Comparison of callus initiation and regeneration frequency for two submerged tolerant rice (Oryza sativa). J. Pharmacy Biol. Sci. 9(1): 74-78.
 
6.
Chandra S., Bandopadhyay R., Kumar V., Chandra R. (2010) Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnol. Lett. 32: 1199-1205.
 
7.
Deb C.R., Imchen T. (2010) An efficient in vitro hardening of tissue culture raised plants. Biotechnology 9: 79-83.
 
8.
Ge X., Chu Z., Lin Y., Wang S. (2006) A tissue culture system for different germplasms of indica rice. Plant Cell Rep. 25: 392-402.
 
9.
Hao G., Du X., Zhao F. (2010) Fungal endophytes-induced abscisic acid is required for avonoid accumulation in suspension cells of Ginkgo biloba. Biotechnol. Lett. 32: 305-331.
 
10.
Hoque E.H., Mansfield J.W. (2004) Effect of genotype and explant age on callus induction and subsequent plant regeneration from root-derived callus of indica rice genotypes. Plant Cell Tiss. Org. Cult. 78: 217-223.
 
11.
Hussain M., Khan H., Bano R., Rashid H., Chaudhry Z. (2010) Protocol optimization for efficient callus induction and regeneration in three Pakistani rice cultivars. Pak. J. Bot. 42(2): 879-887.
 
12.
Ilahi I., Bano S., Jabeen M., Rahim F. (2005) Micropropagation of rice (Oryza sativa L. cv. Swat-II) through somatic embryogenesis. Pak. J. Bot. 37: 237-242.
 
13.
Islam M.M., Wahed S.A., Khan K.U. (2004) Studies on callus induction and regeneration from dehusked rice (Oryza sativa L.) seeds. Plant Tissue Cult. 14(2): 155-160.
 
14.
Karthikeyan A., Pandian S.T.K., Ramesh M. (2009) High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.). Physiol. Mol. Biol. Plants. 15: 391-399.
 
15.
Khatun M.M., Ali M.H., Cruz D.D. (2003) Correlation studies on grains physiochemical characteristics of aroma rice. Pak. J. Biol. Sci. 6: 511-513.
 
16.
Lavanya M., Venkateshwarlu B., Devi B.P. (2009) Acclimatization of neemmmicroshootsadaptable to semi-sterile conditions. Indian J. Biotechnol. 8: 218-222.
 
17.
Lee K.S., Jeon S.H., Kim Y.M. (2002) Optimization of mature embryo based in vitro system for high frequency embryogenesis callus induction and plant regeneration from Japonica rice cultivars. Plant Cell Tiss. Org. Cult. 71: 9-13.
 
18.
Mahajan R., Aslam L., Kousar H. (2013) Effect of growth regulators on in vitro cultures of two basmati rice genotypes: Ranbir Basmati and Basmati 370. Int. J. Pharm. Chem. Biol. Sci. 3(4): 1131-1138.
 
19.
Mahmood., Akhtar M.S. (2006) Efficient embryogenic system from tissue culture of mature embryos for some coarse varieties of rice (Oryza sativa L.). Pak. J. Bot. 38(4): 969-975.
 
20.
Medhabati K., Rajiv D.K., Henary Ch., Dikash Th., Sunitibala H. (2014) Androgenic callus induction of the indica rice hybrid of ChakhaoAmubi and Basmati 370. Int. Res. J. Biol. Sci. 3(4): 73-79.
 
21.
Medina R., Faloci M., Marassi M.A. Mroginski L.A. (2004) Genetic stability in rice micropropagation. Biocell 28(1): 13-20.
 
22.
Muhammad I., Zia M.A., Ali S., Roomi S., Iqbal A., Abbas Z., Ali G.M. (2014) Development of efficient regeneration system in different recalcitrant rice cultivars and expression analysis of putative transgenic plants. Int. J. Agric. Biol. 16: 700-706.
 
23.
Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassy with tobacco tissue culture. Physiol. Plant. 15: 473-497.
 
24.
Naqvi S.S.M., Sultana T., Yasmin T., Mahmood T., Akhtar M.S. (2006) Efficient embryogenic system from tissue culture of mature embryos for some coarse varieties of rice (Oryza sativa L.). Pak. J. Bot. 38: 969-975.
 
25.
Niroula R.K., Sah B.P., Bimb H.P., Nayak S. (2005) Effect of genotype and culture media on callus induction and plant regeneration from matured rice grain culture. J. Inst. Agri. Anim. Sci. 26: 21-26.
 
26.
Noor A., Rashid H., Chaudhry Z., Mirza B. (2005) High frequency regeneration from scutellum derived calli of basmati rice cv. Basmati 385 and Super Basmati. Pak. J. Bot. 37(3): 673-684.
 
27.
Pons M.J., Marfa V., Mele E., Massanger J. (2000) Regeneration and genetic transformation of Spanish rice cultivars using mature embryo. Euphytica 114: 117-122.
 
28.
Puhan P., Siddiq E.A. (2013) Protocol optimization and evaluation of rice varieties response to in vitro regeneration. Adv. Biosci. Biotechnol. 4: 647-653.
 
29.
Rachmawati D., Anzai H. (2006) Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars. Plant Biotechnol. 23: 521-524.
 
30.
Rashid H., Bukhari S.Y.A., Quraishi A. (2001) Callus induction, regeneration and hygromycin selection for rice (Super Basmati). Online J. Biol. Sci. 1(12): 1145-1146.
 
31.
Rashid H., Bokhari S.N.R., Chauhry Z., Naqvi S.M.S. (2003) Studies on genotype response to callus induction from three basmati cultivars of rice (Oryza sativa L.). Pak. J. Biol. Sci. 6(5): 445-447.
 
32.
Sah S.K., Kaur A., Sandhu J.S. (2014) High frequency embryogenic callus induction and whole plant regeneration in japonica rice cv. Kitaake. J. Rice Res. 2(2): 1-5.
 
33.
Sikder M.B.H., Sen P.K., Al-Mamun M.A., Ali R., Rahman S.M. (2006) In vitro regeneration of aromatic rice (Oryza sativa L.). Int. J. Agric. Biol. 6: 759-752.
 
34.
WaniS.H., Sanghera G.S., Gosal S.S. (2011) An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding indica rice (Oryza sativa L.) variety PAU 201. New Biotechnol. 28: 418-422.
 
35.
Xue Q., Earle E.D. (1995) Plant regeneration from protoplasts of cytoplasmic male sterile lines of rice (Oryza sativa L.). Plant Cell Rep. 15: 76-81.
 
36.
Zalc J.M., Wicr H.B., Kidwell K.K., Steber C.M. (2004) Callus induction and plant regeneration from mature embryos of a diverse set of wheat genotypes. Plant Cell Tissue Organ Cult. 76: 277-281.
 
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